中文摘要：

建立 ASE-UPLC（快速溶剂萃取仪-超高效液相色谱）法同时测定不同杜仲制剂中京尼平苷酸（GPA）、绿原酸（CA）、松脂醇二葡萄糖苷（PDG）3种指标成分含量的方法.采用70%甲醇水,温度40℃,压力103.4 MPa 的 ASE 法提取；色谱柱 Acquity UPLC BEH C18（100 mm×2.1 mm,1.7μm）；乙腈（A）-0.1%甲酸水（B）为流动相；流速0.15 mL/min；洗脱梯度：0~15 min,70%B~90%B；检测波长237 nm；柱温20℃.结果显示3种被测成分分别在选定的范围内线性关系良好；精密度、重复性良好,相对标准偏差（RSD ）分别均小于3.0%；平均加标回收率在95.84%~102.69%,RSD 均小于3.0%.表明所建立的方法快捷、准确、重复性好,可用于杜仲制剂中主要成分的含量测定.

英文摘要：

An ASE-UPLC method for simultaneous determination of three components including geniposidic acid,chlorogenic acid and pinoresinol diglucoside in the different preparations containing Eucommia ulmoides was established.The ASE extraction was conducted with 70% MeOH-H 2 O as solvent at 40 ℃ and 103.4 MPa, and UPLC analysis was performed on an Acquity UPLC BEH C18 column （100 nm×2.1 mm,1.7 μm）with acetonitrile （A）-0.1% formic acid （B）as mobile phases for gradient elution （0 ~1 5 min,70% B~90% B）at the flow rate of 0.1 5 mL/min.The detection wavelength was 237 nm and the column temperature was 20 ℃. The results showed that three components were separated completely and showed good linearity,precision and repeatability （RSD ＆lt;3.0% respectively）.Recoveries were between 95.84% ~ 102.69% （RSD ＆lt; 3.0%）.The data suggested the established method was rapid,accurate,good reproducible and could be applied to simultane-ously determine main components in the preparations containing Eucommia ulmoides .