【目的】探讨高浓度CO2对切花菊的瓶插品质、生理生化及结构特征的影响,为高浓度CO2在切花菊乃至其它种类切花保鲜上的应用提供理论依据。【方法】以切花菊品种‘四季黄’切花为材料,瓶插去离子水中后分别置于380(对照)和760(高浓度CO2)μmol·mol-1条件下,利用光合测定、石蜡切片、透射电子显微镜技术及其它一些生理手段,定期研究高浓度CO2对切花保鲜效果、生理及结构特征的影响。【结果】与对照相比,高浓度CO2增加了花枝鲜重,增大了花朵开放直径,延缓了切花菊的衰老进程,延长了瓶插寿命。此外,高浓度CO2提高了切花菊的光合速率、叶绿素和可溶性糖的含量及超氧物歧化酶(SOD)和过氧化物酶(POD)的活性,降低了丙二醛(MDA)的含量。最后,高浓度CO2减缓了叶片和花瓣细胞的降解。【结论】高浓度CO2可能直接通过提高光合速率及碳水化合物含量来间接提高SOD和POD活性而延缓细胞降解,最终延长了切花瓶插寿命。
【Objective】 The effects of elevated CO2 on vase quality, physiological and structural characteristics of cut chrysanthemum were investigated for the purpose of applying elevated CO2 to the fresh-keeping cut chrysanthemum and even other cut flowers. 【Method】 Cut flowers of Chrysanthemum morifolium ‘Sijihuang’ were firstly held into deionized water, and then subjected to 380 (the control) and 760 (elevated CO2) μmol·mol-1 CO2 , respectively. The changes in morphology, physiology and structure were examined using portable photosynthesis system, the technique of paraffin section, transmission electron microscopy, and some other physiological methods. 【Result】 Compared with the control, elevated CO2 increased fresh weight and flower diameter of cut chrysanthemum, retarded its senescence, and extended its vase life. In addition, compared with the control, elevated CO2 enhanced photosynthetic rate, contents of chlorophyll and soluble sugar, and activities of superoxide dismutase (SOD) and peroxidase (POD), but reduced malondialdehyde (MDA) content. Furthermore, elevated CO2 retarded cell degeneration in leaves and petals in comparison to the control. 【Conclusion】 It is possible that elevated CO2 may provide more carbohydrates for normal cell metabolism of cut flowers through directly increasing photosynthetic rate, and indirectly retarded cell degeneration by enhancing the activities of SOD and POD. As a consequence, elevated CO2 extended the life of cut flowers.