中文摘要：

目的三价无机砷通过细胞膜上的水甘油通道蛋白质转运进入细胞内,本研究探讨锌离子对细胞和组织吸收三价无机砷的作用。方法 32只雄性ICR小鼠随机分为4组（8只/组）,灌胃给予ICR小鼠10 mg/kg三氧化二砷前15 min灌胃给予不同浓度的硫酸锌（浓度分别为0、25、50和100 mg/kg）。给予三氧化二砷2 h后,取小鼠肝脏、肾脏、心脏、肺、皮肤和睾丸等组织经硝酸消化后,原子荧光光谱法测定组织中砷含量。培养Raw 264.7细胞,按染毒剂量分为4组：对照组、砷暴露组（iAs^3＋10μmol/L）、砷和锌联合暴露组（iAs^3＋10μmol/L＋Zn^2＋10μmol/L、iAs^3＋10μmol/L＋Zn^2＋50μmol/L）,利用细胞MTT实验检测细胞活性变化。结果给予小鼠50或100 mg/kg硫酸锌和10 mg/kg三氧化二砷后,与仅给予三氧化二砷小鼠相比,肝脏、肾脏、心脏、睾丸和皮肤组织中砷含量明显减低,而低剂量硫酸锌（25 mg/kg）对小鼠组织中砷含量没有影响。不管给予硫酸锌剂量的高低,肺组织中砷含量均没有明显改变。Raw 264.7细胞培养基中加入10和50μmol/L硫酸锌后,砷对细胞的毒性明显降低。结论二价锌离子能抑制多个组织吸收三价无机砷,从而使组织中砷含量降低;二价锌离子也能降低三价无机砷对Raw 264.7细胞的毒性。

英文摘要：

Objective Trivalent inorganic arsenic[iAs^3＋]is transported into cells by aquaglyceroporins in the cell membrane,this study investigated the effect of Zn^2＋ on the uptake of iAs^3＋by tissues of mice and cells. Methods 32 male mice were divided into four groups randomly（ 8 /group）,the four groups were treated with different does of 0 mg·kg^-1,25 mg·kg^-1,50 mg·kg^-1,100 mg·kg^-1 zinc sulphate by intragastric administration,respectively. After 15 minutes,they were all administered 10 mg·kg^-1 iAs^3＋ by intragastric administration. 2 hours later,mice tissues（ liver,kidney,heart,lung,skin,testis and so on） were digested by nitric acid and measured the concentration of arsenic in tissues by atomic fluorescence spectrometer. Raw 264. 7 cells were designed into four groups（ control,10 μmol /L iAs^3＋,10 μmol/L iAs^3＋＋ 10 μmol /L Zn^2＋,10 μmol/L iAs^3＋＋ 50 μmol /L Zn^2＋）,MTT assay was used to detect the toxicity of cells. Results The levels of arsenic in liver,kidney,heart,testis and skin were decreased significantly after treatment with 50 mg·kg^-1 and 100 mg·kg^-1Zn^2＋ compared with mice treated with iAs^3＋only,but the level of arsenic in the lung were no difference among four groups. After Raw 264. 7 cells cocultured with 10μmol/L or 50 μmol/L Zn^2＋,the toxicity of iAs^3＋on cells was decreased. Conclusion Zn^2＋ may inhibit the uptake of iAs^3＋ in tissues of mice,and decreased the levels of As in tissues. Zn^2＋also lowered the cytotoxicity of iAs2 ＋in Raw 264. 7 cells.