中文摘要：

采用聚乙二醇/硫酸铵体系对生物胺氧化酶进行萃取。通过聚乙二醇的分子质量和质量分数、(NH4)2SO4的质量分数、体系p H值以及温度单因素实验,初步确定最优值,再用Box-Behnken响应面进一步优化实验并进行结果分析,得到最佳实验条件为:质量分数为25%的PEG2000、质量分数为10%的(NH4)2SO4、p H值为7.5、温度为30℃,实验的萃取率为96.5%,纯化倍数为2.38。

英文摘要：

PEG/(NH4) 2SO4 aqueous-phase systems was used to extract biogenic amine oxidase that produced by Lactobacillus rhamnosus.Based on the single factor experiments including PEG molecular mass and concentration,ammonium sulfate concentration,pH value and temperature,preliminarily determine the optimum values.Then the design was further optimized with Box-Behnken experiment and the results were analyzed.The results indicated that the best experiment conditions were as follows:PEG molecular mass was 2000,PEG2000 concentration was 25％,ammonium sulfate concentration was 10％,pH value was 7.5,and the temperature was 30 ℃.Under above conditions,the extraction rate was 96.5％ and the purification factor was 2.38.