中文摘要：

客观 Nanoparticles 正在成为指向的药交货的一个重要方法。评估叶酸结合人浆液白朊(HSA ) 的重要性磁性的 nanoparticles (Folate-CDDP/HSA MNP ) ，我们准备了装载药的 Folate-CDDP/HSA MNP 并且描绘了他们的特征。方法第一，叶酸在一个压缩代理人的效果下面与 HSA 被结合，并且结合的率被一个比色的方法用 2 评估， 4， 6 鈥 ? trinitrobenzene 酸性硫酸基的酸。在 N2 气体下面，第二， Fe3O4 磁性的 nanomaterials 被使用传播电子显微镜学(TEM ) 准备并且描绘， SEMedition 和 X 光检查衍射(XRD ) 。最后， Folate-CDDP/HSA MNP 被使用一种溶剂蒸发技术准备。TEM 被用来观察粒子形态学。准备建筑群的粒子尺寸和分发被一个激光粒子尺寸分析器决定。药装载体积和药版本被一个高效液体层析方法(HPLC ) 在 vitro 调查。我们成功地准备了的结果叶酸结合 HSA 和它的结合的率是 27.26 渭 g /mg。在 TEM 下面， Fe3O4 磁性的 nanoparticles 是高度电子密度并且在 10-20 nm 的范围有平尺寸分布。它被 Fe3O4 磁性的 nanoparticles 成功地被准备了的 SEMedition 和 XRD 证实。在 TEM 下面，装载药的磁性的 nanoparticles 被观察，它有圆形状，类似的一致尺寸和光滑的表面。他们的平均尺寸是被激光散布决定的 79 nm，并且他们展出了磁性的应答。封装效率是 89.75% ，有效的药装载是打算的是 15.25% 。在 vitro 的版本结果证明一半在 cisplatin 答案释放 cisplatin 的时间(t1/2 ) ， Folate-CDDP/HSA MNP 分别地是 65 min 和 24 h，它显示 microspheres 有明显的效果支撑版本。结论：Folate-CDDP/HSA MNP 成功地被准备。准备进程和相关特征数据为进一步的学习提供了一个基础，包括在由肿瘤房间的 vivo 和他们的吸入的 nanoparticles 分发的机制。

英文摘要：

Objective： Nanoparticles are becoming an important method of targeted drug delivery. To evaluate the importance of folate-conjugated human serum albumin （HSA） magnetic nanoparticles （Folate-CDDP/HSA MNP）, we prepared drug-loaded Folate-CDDP/HSA MNPs and characterized their features. Methods： First, folate was conjugated with HSA under the effect of a condensing agent, and the conjugating rate was evaluated by a colorimetric method using 2, 4, 6 - trinitrobenzene sulfonic acid. Second, under N., gas, Fe：~O1 magnetic nanomaterials were prepared and characterized by using transmission electron microscopy （TEM）, SEM-EDS and X-ray diffraction （XRD）. Finally, Folate-CDDP/HSA MNP was prepared by using a solvent evaporation technique. TEM was used to observe particle morphology. The particle size and distribution of the prepared complexes were determined by a Laser particle size analyzer. Drug loading volume and drug release were investigated by a high performance liquid chromatography method （HPLC） in vitro. Results： We successfully prepared folate-conjugated HSA and its conjugating rate was 27.26 μg/mg. Under TEM, Fe2O4 magnetic nanoparticles were highly electron density and had an even size distribution in the range of 10-20 nm. It was confirmed by SEM-EDS and XRD that Fe304 magnetic nanoparticles had been successfully prepared. Under TEM, drug-loaded magnetic nanoparticles were observed, which had a round shape, similar uniform size and smooth surface. Their average size was 79 nm which was determined by laser scattering, and they exhibited magnetic responsiveness. Encapsulation efficiency was 89.75% and effective drug loading was calculated to be 15.25%. The release results in vitro showed that the half release time （ta/2） of cisplatin in cisplatin Solution and Folate-CDDP/HSA MNP was 65 min and 24 h respectively, which indicated that microspheres had an obvious effect of sustained-release. Conclusion： Folate-CDDP/HSA MNPs were prepared successfully. The preparation process and re