中文摘要：

目的：研究小鼠肝内B220^＋／DEC205^＋树突状细胞对结肠癌肝转移的影响。方法：小鼠尾静脉大容量快速注射重组质粒plasmid-IL-3／CD40L，诱导肝B220^＋／DEC205^＋树突状细胞增殖，小鼠脾内接种结肠腺癌CT26细胞，检测肝表面转移癌结节数量及脾内癌结节大小和重量、血清癌胚抗原含量及小鼠生存期。结果：处理组小鼠肝非实质细胞较对照组明显增多。小鼠脾包膜下接种CT26细胞后第14天，脾均有癌结节形成，仅肝表面见癌转移结节，转移率为100％。处理组肝表面转移癌结节数量明显多于对照组。脾内癌结节的大小、重量，处理组与对照组比较无明显差别。处理组较对照组血清癌胚抗原水平显著升高，小鼠生存期较对照组缩短。结论：小鼠肝内经重组质粒plasmid—IL-3／CD40L诱导扩增的B220^＋／DCE205^＋树突状细胞对结肠癌肝转移具有促进作用。

英文摘要：

Objective： To explore the effect of mouse liver-derived B220^＋/DEC205^＋ dendritic cells on hepatic metastases from colonic cancer in rico. Methods： Liver-derived B220^＋/DEC205^＋ dendritic cells were harvested after tail vein was injected by recombinant plasmid containing genes encoding IL-3 and CD40L rapidly with large dose in BALB/C mice. After intrasplenic inoculation with colon adenocarcinoma CT26 cells, the number and size of metastatic carcinoma nodules on mice liver surface were observed and counted. The size and weight of implanted carcinoma in the spleen, the serum level of carcinoembryonic antigen （CEA） and the survival time were detected. Results： Liver nonparenchymal cells dramatically increased in the treated group, but not in the control group. The mice were evaluated at the 14 days after intrasplenic inoculation. All mice loaded splenic implanted carcinoma and developed liver metastases, but other organic metastases were not observed. The incidence of liver metastasis was 100% in the intrasplenic inoculation models. The number of metastatic carcinoma nodules on liver surface in the treated group was more than that in the control group. There was no significant difference in splenic carcinoma size and weight in the treated group and control group. The serum CEA level increased significantly in the treated group as compared with the control group. Compared to the control group, the survival time was shortened in the treated group. Conclusion ： Mouse liver-derived B220^＋/DEC 205^＋ dendritic cells expanded by plasmid-IL-3 and CD40L genes may play a role on the liver metastasis of colon carcinoma.