中文摘要：

以割手密GXS85-30×GXS87-16的杂交后代为材料,应用目标起始密码子多态性（SCoT）分子标记对杂交后代进行杂种鉴定,获得由157个单株组成的F1杂种群,同时对比SCoT、AFLP和SSR分子标记在割手密基因组多态性分析和获得分离标记的效果,证实SCoT在扩增DNA多态性上优于SSR分子标记,在获取分离标记上优于AFLP分子标记,验证了SCoT分子标记技术在割手密遗传分析中的应用效果,为割手密遗传分析和遗传图谱构建提供了一种新型高效的目的基因分子标记技术。

英文摘要：

To assess the effectiveness of the new SCoT molecular marking technique in Saccharum spontaneum L.,157 offsprings of the crosses between Saccharum spontaneum（GXS85-30） and Saccharum spontaneum（GXS8716） were analyzed by SSR,AFLP,and SCoT molecular marking technique respectively.Comparatively,SCoT molecular marking technique was better than SSR molecular marking technique in amplification of polymorphic DNA,and had better segregation marker than AFLP molecular marking technique.These results demonstrated that SCoT molecular marking technique had potential application prospects in genetic analysis and genetic linkage map construction of Saccharum spontaneum L.