为了探讨短期饥饿胁迫对刀鲚(Coilia nasus)相关生理学指标的影响,以室内养殖刀鲚为实验对象,研究20 d的短期饥饿对其形体、体成分和血液生化指标的影响,实验组分别在第0、2、5、10、15和20天取样。结果表明,脏体比和肝体比在饥饿的第2天即显著下降(P〈0.05),肥满度和体质量在20 d的饥饿期内呈下降趋势,但变化不显著(P〉0.05)。刀鲚肌肉粗脂肪在第5天显著下降(P〈0.05),下降5.99%,而粗蛋白含量在第15天时才显著降低(P〈0.05),水分在整个饥饿期间总体呈上升趋势,第10天显著上升(P〈0.05),粗灰分含量在饥饿期间变化不显著(P〉0.05)。血清总蛋白、球蛋白、胆固醇和皮质醇随着饥饿时间的延长呈现先升高后降低的趋势,而血清甘油三酯和血糖总体呈先急速下降后维持一定水平,其中甘油三酯在饥饿至第2天显著下降(P〈0.05),血糖浓度在第5天显著下降(P〈0.05)。表明短期饥饿胁迫使刀鲚形体发生一定的变化,随着饥饿时间的延长,刀鲚首先动用体内储存的脂肪来满足能量供应,再动用蛋白质来维持体内的正常代谢,血液生化指标也发生与饥饿相适应的变化。
The impact of starvation stress on morphological change, chemical composition and blood biochemistry of the Coilia nasus was studied using hatchery reared fish. Individuals were sampled at 0( control),2,5,10 and 20 days of starvation. Tables 1- 3 showed that viscerosomatic and hepatosomatic indices significantly declined from the 2ndday( P 〈 0. 05); the relative fatness and body mass exhibited a declining trend after 20 days,but without significant difference( P 〉 0. 05). The crude fat content in fishmuscle of C. nasus declined significantly after 5 days with the change of 5. 99%( P 〈 0. 05),while the amount of crude protein showed significant decline after 15 days( P 〈 0. 05). In contrast,water in fish muscle gradually increased from day 1 and reached significantly different level after 10 days( P 〈 0. 05). The crude ash content did not have significant change during the experimental duration( P 〉 0. 05). The contents of serum total protein,globulin,cortisol and cholesterin showed a positive correlation at the beginning of experiment and exhibited a negative correlation thereafter. The content of serum triglycerides significantly deceased after 2 days( P 〈 0. 05),while the content of glucose significantly deceased after 5 days( P 〈 0. 05),and both then reached a stable low level. Our results indicated that the C. nasus could experience morphological change under the circumstance of starvation. They would utilize stored fat components to provide energy support,and then maintain the regular body metabolism using proteins. Their blood biochemical parameters had corresponding change to the degrees of starvation.