中文摘要：

摘要揭示了电离辐射对成骨细胞系MC3T3细胞的增殖及其对Notchl和Jaggedl基因表达的影响。成骨细胞系MC3T3细胞，经过0、2和4Gy^137Csγ射线照射，运用MTT、实时定量PCR技术，检测电离辐射对与MC3T3细胞的增殖和增殖分化相关基因Notchl和Jaggedl表达的影响。照射剂量为2和4Gy时，细胞的增殖下降（p〈0．05，p〈0．01）；照射剂量为2Gy时，Notchl基因表达量降低（p〈0．05）；照射剂量为2和4Gy时，Jaggedl基因的表达量均降低（p〈0．01，p〈0．05）。电离辐射损伤使成骨细胞系MC3T3的增殖能力下降，同时诱发Notchl基因和Jaggedl的低表达。

英文摘要：

ABSTRACT In order to investigate the effects of ionizing radiation on MC3T3 cells＇ proliferation and expression changes of Notchl and Jaggedl genes, MC3T3 cells were exposed to γ-rays with 0, 2 and 4 Gy, respectively. MTT assay and real-time PCR were adopted to detect the proliferation of MC3T3 cells and the changes of expression of Notchl and Jaggedl genes which could be in association with proliferation and differentiation. When exposed to 2 and 4 Gy γ-rays irradiation, the MC3T3 cells＇ proliferation reduced （p〈0.01, p〈0.01）. The expression of Notchl decreased when treated with 2 Gy irradiation （p〈0.05）. The expression of Jaggedl regulated down when given 2 and 4 Gy irradiation （p〈0.01, p〈0.05）. Irradiated MC3T3 cells＇ proliferation potentialities reduced and Notchl and Jaggedlwere regulated down.