中文摘要：

目的对成年斑马鱼进行邻苯二甲酸二丁酯（DBP）急性染毒，观察其胚胎发育过程，探索DBP对其胚胎发育的影响。方法选取健康成年斑马鱼80条，雌雄各半，适应性分开饲养1个月后，分别分成4组，给予1250μg／L和625μ／LDBP经水染毒，同时设溶剂（0．01％丙酮）对照组和空白对照组。染毒7天后，按雌雄1：1的比例交配，在倒置显微镜下观察整个胚胎发育过程，并计算受精率、72h死亡率、仔鱼72h孵化率及出生体重和身长。结果各组的2h受精率的大小如下：空白对照组〉丙酮对照组〉625μg／LDBP染毒组〉1250μg／L DBP染毒组，分别为98．09％、95．76％、95．31％和94．42％。而各组的72h死亡率分别为18.29％、23.34％、49．45％和72．41％，72h孵化率分别为96．43％、82．37％、48．11％和26．79％。染毒组的死亡率高于非染毒组，孵化率低于非染毒组。与正常组和丙酮对照组相比，1250μg／L和625μg／LDBP染毒组胚胎发育明显迟缓，仔鱼身长明显变短，并有统计学差异。暴露剂量越大，身长越短。结论625μg／L以上剂量的DBP染毒能导致斑马鱼胚胎发育迟缓。

英文摘要：

Objective To investigate the embryotoxicity of Di-n-butyl phthalate （DBP） on zebrafish. Methods In this study, 80 healthy adult zebrafish were selected, containing 40 female and 40 male. After separated raised for one month, they were separated 4 groups. Each group was exposed to 1250μg/L DBP（dissolved in 0.01% acetone）, 625μg/L DBP （dissolved in 0.01% acetone）, 0.01% acetone and water, respectively. Acetone and water were used as solvent control and blank control, respectively. After exposed to DBP for seven days, zebrafish were mated at the ratio of 1 ： 1 （male to female）. Embryo development, fertility rate, 72h death rate, 72h hatching ratio, body weight and length of zebrafish were observed under the inverted microscope and by using microbalance and callipers. Results The fertility rates of the blank group, acetone control group, 625μg/L DBP-treated group and 1250μg/L DBP-treated group were 98.09% ,95.76% ,95.31% and 94.42%, respectively. And the 72h death rates of various groups were 18.29%, 23.34% ,49.45% and 72.41%, respectively. The hatching ratios were 96.43% ,82.37% ,48.11% and 26.79%. The 72h death rates of DBP-treated groups were higher than those of non-treated groups, but the hatching ratios were lower. Compared with the blank and acetone control group, retardation of embryo development in DBP-treated groups were observed obviously. Although no significant differences were observed on body weight, the body length were significant shorter in DBP-treated groups, while comparing with blank and acetone control group. The higher dose zebrafish exposed, the less body length the young had. Conclusion 625μg/L and higher DBP treated may be the cause of the retardation of zebrafish embryo development.