中文摘要：

目的：为有效地控制预防流行区犬科动物的棘球绦虫感染情况，建立一种快速检测棘球绦虫感染犬的粪便DNA检测方法-环介导等温扩增技术（loop-mediated isothermal amplification ．LAMP）。方法针对细粒棘球绦虫线粒体DNA ND2基因6个位点特异性的设计4条LAMP引物，利用LAMP法和普通PCR方法同时检测棘球绦虫、肥胖带绦虫以及犬肠道内的其它寄生虫DNA来验证LAMP方法的特异性；将转入ND2基因片段的质粒作为标准阳性对照，并做梯度稀释后同时用 LAMP和 PCR方法进行检测比较两者的敏感性。此外，将采集的46份犬粪便标本提取DNA ，分别运用LAMP和犬尸体剖检进行感染犬的初步检测评估。结果 E ．g mtDNA ND2基因的LAMP引物能够鉴别多房棘球绦虫和细粒棘球绦虫这两个相近的种属，并不与其它待检寄生虫发生交叉反应，且最低检测限度为4×10^1拷贝（灵敏度比普通PCR高出10^3倍）。在初步对46份犬粪便DNA检测结果中 ND2 LAMP引物显示出较好的灵敏度和特异度，χ2检验结果该方法与犬尸体剖检方法的诊断效果无统计学差异。结论本研究初步建立了LAM P技术检测细粒棘球绦虫感染犬的新方法，在各项特异度、灵敏度和样本检测中展现出较好的效果。该方法不需要昂贵的仪器设备和繁琐的电泳分析过程，有望成为流行区和基层兽医站细粒棘球绦虫感染犬检测的新方法。

英文摘要：

To control and prevent the Echinococcus in the place ,we established a loop-mediated isothermal amplification （LAMP） assay for rapid detection of Echinococcus species specific DNA from dog faeces .Four primers which recognizing 6 dis-tinct regions on the NADH dehydrogenase subunit 2 （ND2） gene of Echinococcus granulosus were designed and used for LAMP assay .The specificity of LAMP assay was evaluated using DNA extracted from Echinococcus granulosus , Taenia saginata , and other dog intestinal parasites .In addition ,the sensitivity of LAMP assay was compared with that of conventional PCR using recombinant plasmid carrying Echinococcus granulosus ND2 gene fragment as standard template DNA after 10-fold serial dilution .Furthermore ,we extracted DNA from 46 canine fecal samples collected from endemic areas ,and tested the copro-DNA samples using LAMP and necropsy method .Results showed that E .g ND2 primer sets could differentiate Echinococcus granulosus from Echinococcus multilocularis without cross reaction among other parasites detected .Furthermore ,the LAMP assay with primer sets to the ND2 gene could detect 4 × 10^1 copies of target gene ,demonstrating 10^3 times higher sensitivity than that of conventional PCR methods .The LAMP assay with primer set to ND2 gene showed good sensitivity and specificity to detect copro-DNA samples extracted from fecal samples of 46 dogs tested in endemic areas .There was no statistically signifi-cant difference among LAMP and necropsy .In this study ,a sensitive ,specific and rapid copro-DNA detection LAMP assay was developed successfully for diagnosis of dogs infected with Echinococcus granulosus .Due to its rapidity ,simplicity ,speci-ficity and sensitivity ,the LAMP assay is a promising new tool for rapid detection of dogs infected with Echinococcus spp .dur-ing the field survey or in poor-equipped laboratories .