根据光的散射和透射理论,设计了一种用光纤检测微量样本悬浮颗粒浓度的装置。该装置采用了散射和透射两束光纤,且散射角度可调旨在寻找最优的测量角度。实验选用波长为785 nm的激光作为光源,在室温条件下,对酵母菌浓度测量进行了实验研究并用散透比和透射光两种方式处理数据。结果表明,酵母菌浓度为0~5%时,散透比与浓度呈近似线性关系;酵母菌浓度为5%~10%时,透射光与浓度呈近似线性关系,测量的误差范围小于±0.2%。该装置能实现微量样本的快速检测,在微机电系统(MEMS)领域具有较好的应用前景。
Based on the theory that the varying of concentration leads to certain scattering and transmitting,an equipment was designed with two beams of optical fibers that one was for scattering and the other was for transmitting.The scattering angle was adjustable in order to find the optimal type of detection and the method with ratio of scattering to transmitting was used.Under the room temperature circumstance,the concentration of yeast was tested by using a 785 nm laser.The result indicated that 0~5%,there was nearly a linear relationship between ratio of scattering to transmitting and concentration;for 5%~10%,and there was nearly a linear relationship between transmitting and concentration.Besides,the measured repeatable maximum error was less than ±0.2%.The equipment enabled rapid measurement of small sample and had a good application prospect in the field of MEMS.