采用共生培养方法研究菖蒲(Acoruscalamus)和铜绿微囊藻(Microcystisaeruginosa)之间的化感作用.结果表明,初始密度为1.0×10^7indmL^-1的铜绿微囊藻与20gL^-1菖蒲共生培养时,菖蒲对铜绿微囊藻在一定程度上产生了化感抑制,但是铜绿微囊藻的生长作用占优势,d20抑制率仅为16%.而在培养过程中,菖蒲叶绿素a含量逐渐减少,d20叶绿素a含量仅为对照组的27%;超氧化物歧化酶(SOD)活性在d12开始明显降低,d20仅为对照组的46%;丙二醛(MDA)含量呈现逐渐增加趋势,d20为对照组的172%;而生物量仅为对照组的62%.研究表明高密度铜绿微囊藻可以诱导菖蒲产生氧化胁迫,引起细胞结构严重损伤,从而强烈抑制菖蒲的生长.图4参31
Acorus calamus co-cultivated with M. aeruginosa was used to study the mutual allelopathy of A. calamus and M. aeruginosa. The results showed that, 20 g L^-1 of A. calamus inhibited to some extent the initial density 1.0×10^7indmL^-1 of M. aeruginosa, and the growth of M. aeruginosa gained dominance, which resulted in that IR was only 16% after 20-day co- cultivation. But, during the whole process of incubation, the chlorophyll-a content of A. calamus declined gradually and it was only 27% compared with the control group after 20-day incubation. SOD activity of A. calamus reduced on the 12th day, and the activity was only 46% compared with the control group after 20-day incubation. MDA content of A. calamus however increased gradually from the beginning, and the content was 172% compared with the control group on the 12th day. And the biomass of A. calamus was only 62% of that in the control group after 20-day incubation. It suggested that high density of M. aeruginosa could induce oxidative stress to A. calamus, which could cause serious damage on cell structures and strongly inhibit the growth ofA. calamus. Fig 4, Ref 31