中文摘要：

目的研究巨噬细胞诱导性C型凝集素样受体（MINCLE）在健康者、类风湿关节炎（RA）和骨关节炎患者外周血及关节滑液中的表达特征，探讨其与RA发病的相关性。方法①采用实时定量聚合酶链反应（PCR）方法在mRNA水平检测RA患者（253例）及健康人（71名）外周血单个核细胞（PBMC）中MINCLE的表达；②采用流式细胞术在蛋白水平检测RA患者（18例）、骨关节炎患者（5例）及健康人（12名）外周血及关节滑液巨噬细胞、髓样树突状细胞（mDC）和浆细胞样树突状细胞（pDC）中MINCLE的表达；采用Mann—WhitnevU检验或配对t检验统计分析MINCLE在健康者、骨关节炎患者和RA患者外周血及关节滑液之间的表达差异。结果①在mRNA水平，外周血PBMC中RA患者的MINCLE表达量高于健康对照组[（1．65±0．36）和（0．37±0．06），U=6057，P=2．75×10^-5]；②在蛋白水平，健康人及骨关节炎患者外周血巨噬细胞、mDC及pDC中未见MINCLE的表达；在骨关节炎关节滑液中，MINCLE仅低水平表达于mDC[（7．5±2．9）％]中，而MINCLE在RA患者关节滑液mDC中呈高表达[（34．8±4．4）％，U=0，P=2．6×10^—3]；MINCLE在RA患者的滑液巨噬细胞及mDC的表达均明显高于其在相对应的外周血中的水平[巨噬细胞（2．01±0．53）％和（0．27±0．51）％，t=4．879，P=2．23×10^-6；mDC为（34．8±4．4）％和（21．7±5．5）％，t=2．535，P=0．017]。结论MINCLE的表达特征具有RA组织特异性，MINCLE很可能为RA发病的一个重要标记物。

英文摘要：

Objective To determine the expression pattern of macrophage-inducible c-type lectin （M1NCLE） on Macrophage （Mφ）, myeloid dendritic cell （mDC） and plasmacytoid DC （pDC） in peripheral blood （PB） and synovial fluid （SF） in patients with rheumatoid arthritis （RA）. Methods For mRNA expression of MINCLE, 253 RA patients and 71 healthy control subjects were enrolled. The mRNA level of MINCLE was determined by real-time PCR. For protein expression of MiNCLE, 18 patients with RA, 5 patients with osteoarthritis （OA） and 12 healthy control subjects were enrolled. The expression of MINCLE on Mφ, mDC and pDC were detected by flow cytometry. The differences of MINCLE expressions in PB between RA patients, OA patients and healthy controls, or differences between PB and SF in RA patients were analyzed＇using Mann-Whitney U test or paired-samples t test. Results ① Compared to the healthy controls, RA patients showed elevated mRNA expression level of MINCLE in PBMCs [ （1.65±0.36） υs （0.37±0.06）, U=6057, P=-2.75×10^-5]. ② At protein level, MINCLE was hardly detected in Me, mDC and pDC in PB of OA patients and healthy controls. In SF, MINCLE was highly expressed on mDC in RA patients, compared with that in OA patients [（34.8±4.4）%, U=0, P=2.6×10^-3]. In RA patients, the expression level of MINCLE was remarkably elevated in Mφ, mDC and pDC in SF compared with that in PB [ Mφ （2.01±0.53）% υs （0.27 ±0.51）%, t =4.879, P=-2.23×10^-6; mDC（34.8±4.4）% υs （22.7±5.5）%, t=2.535,P=0.017]. Conclnsion MINCLE is selectively expressed on Mφ, mDC and pDC in SF in RA patients. MINCLE may serve as a potential important marker, or even target, for RA and possibly even for inflammation in general.