中文摘要：

体外试验利用甲型流感病毒感染狗肾细胞（MDCK）致细胞病变（CPE）模型评价苹果多酚对流感病毒复制的抑制作用;体内试验利用小鼠感染流感病毒致鼠肺适应株模型,以死亡保护率和生命延长率等为指标,评价苹果多酚体内抗流感病毒作用。利用体外不同作用模式试验、不同作用时间点试验和血凝抑制试验（HI）研究苹果多酚抗流感病毒作用机制。研究结果表明,苹果多酚对甲型H9N2、H3N2和H1N1 3种亚型流感病毒引起的细胞病变表现出不同程度的抑制作用,50%抑制浓度（IC50）分别是0.012、0.0042、0.003 mg/mL。200 mg/（kg.d）剂量苹果多酚对H1N1亚型流感病毒感染小鼠有明显的保护作用和延长生命率的作用（P〈0.05）。苹果多酚对3种亚型流感病毒的血凝抑制最小浓度（MIC）在0.25～0.5 mg/mL范围之间。对病毒复制周期的吸附和穿入的抑制作用是其主要作用机制。说明苹果多酚在体内外均具有显著的抗流感病毒作用。

英文摘要：

Viral cytopathic effect（CPE） was used to evaluate anti-influenza activity of apple polyphenols in vitro.Mouse infection model was used with life-protection rate as parameters to indicate inhibitory effect of apple polyphenols on influenza virus in vivo.Mechanism of action of apple polyphenols in detail was studied by action modes study,time of addition study and HI assay.The results showed that apple polyphenols exhibited inhibitory effect at various degree against a spectrum of influenza A（H9N2,H3N2 and H1N1）.50% inhibitory concentration（IC50） were 0.012,0.0042 and 0.003 mg/mL,respectively.Apple polyphenols at 200 mg（kg·d） significantly improved the survival rate and prolonged the life span of mouse infected with influenza virus H1N1.Apple polyphenols inhibited absorption of the viruses on red blood cell with minimum inhibitory concentrations（MIC） ranging from 0.25 to 0.5 mg/mL.Inhibition of virus absorption and internalization by endocytosis was the main mechanism of action of apple polyphenols.The study showed that apple polyphenols exhibited significant inhibitory effect against influenza virus in vivo and in vitro.