中文摘要：

目的：研究大鼠脑出血后血肿周围组织葡萄糖载体蛋白-1（GLUT1）表达的动态变化规律及头穴透刺的治疗作用。方法：将雄性Wistar大鼠随机分为空白组、假手术组、模型组、针刺组，每组又分为24h、72h、168h三个小组．采用胶原酶／肝素联合注入法制备脑出血大鼠模型，给以百会透太阳穴电针治疗，各组动物在指定时间断头取脑．取血肿周围3mm范围内组织，采用免疫组织化学法观测GLUT1表达的变化。结果：模型组：三个时间点均见大部分GLUT1阳性微血管形态变窄、皱缩、闭塞，越靠近病灶越严重；GLUT1表达24h先代偿性升高．与假手术组比较P〈0．05，后72h、168h失代偿性降低，与假手术组比较P〈0．05；针刺组：仅在24h时血肿周围出现了轻微的管腔狭窄．之后大部分血管表现为管腔呈圆形扩张，GLUT1在72h、168h也出现下降（P〈0．05），但明显好于模型组（P〈0．05）。结论：针刺在多时间点对GLUT1均有治疗作用，能延长治疗时间窗，减轻病变程度且早期作用明显。

英文摘要：

Objective： To prove the effect of acupuncture through an experimental study of scalp-acupuncture on the express of GLUT1 after intracerebral hemorrhage in rats. Method： Wistar rats were divided into 4 groups： normal group, sham group, model group, acupuncture group. The last three group was divided into three time groups（24h,72h,168h）, The model of intracerebral hemorrhage in rat was made with Collagenase Ⅶ/heparini infusion, and treated with electro-acupuncture of Baihui-Taiyang points, Immunohistochemical method used to observe the expression of GLUT1 around the hemotoma. Result： The model group after intracerebral hemorrhage： morphology of majority positive GLUT1 capillary vessels showed narrowing, shrinking and obliteration in three time points specially near the focus of lesion. The expression of GLUT1 in 24h revealed a compensatory rising at first, compared with control group （P〈0.05）; then lowered, in 72h and in 168h （P〈0.05）; Acupuncture group： in perihemotoma area, the morphology of positive GLUT1 capillary vessels showed slightly narrowing only in 24h, then most of the vessels showed spherical distending, GLUT1 decreased in 72h and 168h （P〈0.05）,but better than the model group（P〈0.05）. Conclusion： Acupuncture have therapeutic effects on three time points, delay the development of pathologic change in time,lengthen the therapy time window, and ohviously effect in the earlier period.