中文摘要：

目的探讨蒺藜皂苷的抗抑郁作用及机制。方法采用孤立大鼠加慢性轻度不可预见性应激方法造抑郁大鼠模型,进行开放旷场,悬尾行为学测试,用LC-MS/MS液质联用法检测大鼠血液中5-羟色胺（5-HT）、γ氨基丁酸（GABA）、去甲肾上腺素（NE）、谷氨酸（Glu）含量变化。实时定量PCR检测海马吲哚胺2,3-双加氧酶（IDO）、核转录因子-κB（NF-κB）、炎性细胞因子白介素1β（IL-1β）基因表达。Western blot检测海马中吲哚胺2,3-双加氧酶（IDO）、细胞因子NF-κB（p65）蛋白水平表达。结果与空白组比较,模型组小鼠旷场运动距离、站立次数和悬尾不动时间明显增加（P〈0.05）,血液中5-羟色胺明显下降（P〈0.05）,海马中IDO、NF-κB、IL-1β基因表达上调,IDO、NF-κB蛋白水平明显上调（P〈0.05）。与模型组比较,蒺藜皂苷给药组能明显减少慢性应激导致大鼠旷场运动距离、站立次数和悬尾不动时间（P〈0.05）。蒺藜皂苷给药组能明显提高大鼠血液中5-羟色胺含量（P〈0.05）,上调海马中抗炎因子IL-10基因表达,明显下调IDO、IL-1β基因表达（P〈0.05）,明显下调IDO蛋白表达水平（P〈0.05）。结论蒺藜皂苷能明显改善动物行为学,具有抗抑郁作用,其作用可能与提高血液中5-羟色胺水平,下调色氨酸前体代谢酶吲哚胺2,3-双加氧酶（IDO）、IL-1β基因表达和降低吲哚胺2,3-双加氧酶（IDO）蛋白表达水平有关。

英文摘要：

Aim Tostudytheantidepressanteffectand mechanism of Gross saponins of Tribulus terrestris. Methods Themodelofdepressionwasestablishedby unpredictable chronic mild stress（UCMS）,then open filed test （OFT）and tail suspension test （TST）were used to evaluate the behavioral changes.LC-MS/MS method was employed to measure blood neurotransmit-ters.mRNA expressions of IDO,IL-10 and IL-1βwere detected by quantitative PCR method.Hippocampus protein expression was detected by Western blot.Re-sults Comparedwithcontrolgroup,modelgroup＇s total distance,number of standing and tail suspension fixed time increased significantly （P 〈0. 05 ）,Neuro-transmitter level of 5-HT in the blood was significantly decreased（P〈0. 05 ）.mRNA expression of IDO and IL-1βwas increased in hippocampus.Protein expres-sion of IDO was significantly increased in hippocampus （P 〈0. 05 ）.Compared with model group,the treat-ment group was significantly decreased in total distance,number of standing and tail suspension fixedtime（P〈0. 05）.Neurotransmitter level of 5-HT in the blood and mRNA expression of IL-10 in hippocampus were significantly increased after treatment （P 〈0. 05 ）.mRNA and protein expression of IDO were ob-viously down-regulated in hippocampus （P 〈0. 05 ）. Conclusions GrosssaponinsofTribulusterrestriscan obviously improve rat behavior and show antidepressanteffect,which can increase neurotransmitter level of 5-HT in the blood,down-regulate mRNA expression of IDO and IL-1β,and obviously increase protein expres-sion levels of IDO in hippocampus（P〈0. 05 ）.