中文摘要：

目的通过对BALB／C小鼠原位结肠癌实验模型应用5’-脱氧氟尿苷治疗，并同传统抗癌药物5-氟尿嘧啶进行比较，观察分析5’-脱氧氟尿苷在动物体内抗结肠癌细胞效应及在组织和血液中的转化。方法60只BALB／C小鼠采用盲肠原位瘤块接种法建立小鼠结肠癌CT26实验模型，术后第3天开始用随机数字表法把小鼠分成3组，每组20只。根据体重分别连续5d腹腔注射：（1）5’-脱氧氟尿苷组：5’-脱氧氟尿苷0．1mg／g；（2）5-FU组：5-FU0．02mg／g；（3）对照组：0．9％氯化钠溶液0．4mL。每组分别于术后第2、3周处死6只小鼠，测量肿瘤重量和体重变化，检测血常规和肝肾功能，并各保留8只小鼠等待自然死亡记录带瘤生存时间。另外把14只小鼠皮下接种CT26细胞约2周后，待注射局部形成皮下肿物，用随机数字表法分成2组，每组各7只，分别腹腔注射5’-脱氧氟尿苷0．1mg／g和5-氟尿嘧啶0．02mg／g，15min后同时处死取血和肿瘤组织测定转化后的5-氟尿嘧啶含量。结果5’-脱氧氟尿苷组小鼠肿瘤重量最小，术后2周和3周平均重量分别为（0．07±0．12）g和（0．24±0．29）g；平均带瘤生存期为（32．6±8．9）d。5-氟尿嘧啶组术后2周和3周平均肿瘤重量分别为（0．74±0．43）g和（1．13±0．75）g，平均带瘤生存期为（22．8±5．9）d。对照组术后2周和3周平均肿瘤重量分别为（0．70±0．47）g和（1．93±0．83）g，平均带瘤生存期为（17．5±2．8）d。5’-脱氧氟尿苷组小鼠肿瘤重量、带瘤生存期与5-氟尿嘧啶组及对照组相比差异具有统计学意义（P〈0．05）。各组小鼠在WBC、Hb、Plt、肝肾功能方面差异无统计学意义（P〉0．05），但5-氟尿嘧啶组在手术及应用药物后体重下降比较明显，与5’-脱氧氟尿苷组及对照组相比差异具有统计学意义（P〈0．01）。5’-脱氧氟尿苷组小鼠在注药15min

英文摘要：

Objective To evaluate the anticancer activity of 5＇-dexoxy-fluorouridine on colon cancer experimental models in BALB/C mice, compared with 5＇-fluorouraeil, an anticancer agent widely used in clinic, meanwhile, examined the conversion of 5＇ - Dexoxy-fluorouridine to 5＇ -fluorouracil in cancer tissues and serum of mouse models. Methods The xenografts of mouse colon cancer cell line CT 26 were trans- plantated to cecum in 60 male BALB/C mice. Three days lated, these mice were divided into 3 groups and intro- peritoneally injected ： （1） 5＇ - dexoxy- fluorouridine 0.1 rag/g, （2） 5＇ - Fluorouracil 0.02 mg/g, （3） 0.9% sodium chloride 0.4 mL （as a control） , respectively. Two and three weeks later, 6 mice were sacri- ficed in every group respectively to measure the weight of tumors and bodies, to examine the Hb, RBC, WBC, PLT, AST, ALT, UREA, and CREA in blood. The rest 8 mice in each group were fed generally, and the survival time from operation to natural death was recorded. In addition, 14 mice with xenografts of CT 26 about 2 weeks, were divided into 2 groups averagely, 5＇ -dexoxy-fluorouridine 0.1 mg/g and 5＇ -flu- orouracil 0.02 mg/g were intro-peritoneally injected respectively. Fifteen rain later, the converted 5 ＇ -flu- orouracil was detected from the blood and tumor tissues in sacrificed mice. Results The lest tumor average weight was found in the mice injected 5＇-dexoxy-fluorouridine, being （0.07±0. 12） g and （0.24g±0.29） g for the mice sacrificed at 2 and 3 weeks later, respectively. The average survival time for rest mice was （32.6±8.9） d. The average tumor weight in 5＇ - fluorouracil group was （0.74±0.43） g and （1.13±0.75） g at 2 and 3 weeks later, and the average survival time for the rest was （22.8±5.9） d, respectively. The average tumor weight in the control group was （0.70±0.47） g and （1.93±0.83） g at 2 and 3 weeks, and the average survival time for the rest was （17.5±2.8） d. Either the average tumor weight or average s