中文摘要：

目的研究As2S2对卵巢癌耐药株C13K/DDP细胞增殖抑制和诱导凋亡的作用。方法以不同浓度（4、6、8、10μmol/L）的As2S2,分三个时间点（24、48、72h）干预C13K/DDP细胞,采用四甲基偶氮唑蓝（MTT）法检测As2S2对C13K/DDP细胞的增殖抑制率;流式细胞仪（FCM）检测细胞凋亡率;Western blot检测BCL-2、BAX、AKT的表达。结果 MTT结果显示不同浓度（4、6、8、10μmol/L）的As2S2作用C13K/DDP细胞后,与DDP组相比其增殖受到抑制,作用呈明显的时效和量效关系,差异有统计学意义（P〈0.01）;流式细胞仪的结果显示6、8μmol/LAs2S2诱导细胞的24h凋亡率分别为（16.05±2）%、（22.30±3）%,DDP组为（9.45±2）%,对照组为（7.82±1.2）%;6、8μmol/LAs2S2诱导细胞48h凋亡率分别为（28.94±1.8）%、（37.85±3）%,DDP组为（14.74±3.2）%,对照组为（9.80±2.6）%,各组比较,差异有统计学意义（P〈0.05）;Western blot结果显示BCL-2、AKT表达下调,BAX表达明显上调。结论 As2S2对人卵巢癌耐药株C13K/DDP细胞具有增殖抑制和诱导凋亡的作用,可能与BCL-2下调、BAX上调或AKT下调有关。

英文摘要：

Objective To investigate the role of As2S2 on C13K/DDP cells proliferation and apoptosis in vitro.Methods C13K/DDP cells were incubated with different concentration of As2S2（4,6,8,10μmol/L）at various periods（24,48,72h）.The cell growth was measured by MTT.Apoptosis was detected by double staining flow cytometry（FCM）.The expression of BCL-2,BAX and AKT was examined by Western blot analysis.Results Compared with DDP group,the proliferation of C13K/DDP cells treated with As2S2 was significantly inhibited in dose-and time-dependent manner（P0.01）.FCM analysis showed that As2S2could markedly induce C13K/DDP cells apoptosis.The apoptotic rates of C13K/DDP cells treated with As2S2（6,8μmol/L）after 24h and 48h were（16.05±2）%,（22.30±3）%and（28.94±1.8）%,（37.85±3）%respectively,there was significant difference compared to control group[（7.82±1.2）%,（9.80±2.6）%]and DDP group[（9.45±2）%,（14.74±3.2）% ]（P0.05）.BCL-2 and AKT expression was down-regulated by As2S2and BAX expression was up-regulated by As2S2.Conclusion As2S2could inhibit the proliferation of C13K/DDP cell and induce cell apoptosis,which may be related to the BCL-2 or AKT down-regulation and BAX up-regulation.