中文摘要：

从连续运行的UASB反应器厌氧污泥中分离得到一株脱氮硫杆菌T.d.a,采用分批摇床试验,采用脱氮硫杆菌标准培养基,以硫代硫酸钠为硫源,研究pH值、温度、氮源（NO3^-N,NH4^＋-N）、能源（S2O3^2-）、碳源（HCO3）、葡萄糖、无机盐（P,Mg^2＋,Fe^2＋）对该菌株自养反硝化的影响.结果表明,在pH6.5～8.0,温度20～35℃的范围内,T.d.a对NO3^-N均有较高的去除速率,其最佳反硝化pH值为7.04,温度为27.40℃.T.d.a对〈416mg/L的NO3^-N有较好的适应性,〉554mg/L的NO3^-N对T.d.a有一定的抑制作用;T.d.a反硝化所需NH4＋-N的限制浓度为2.62mg/L;S2O3^2-浓度对T.d.a反硝化的影响主要取决于其与NO3^-的比例关系,在NO3^-过量的情况下,NO3^-N去除率与加入的S2O3^2-量成近似的正比关系.T.d.a以HCO3^-作为无机碳源时其限制浓度为29.05mg/L;0～2000mg/L的葡萄糖对NO3^-N去除率没有明显影响.P和Mg^2＋的限制浓度分别为0.034,0.059mg/L,Fe^2＋的限制浓度低于0.058mg/L.

英文摘要：

A strain of Thiobacillus denitrificans （T.d.a） was isolated from anaerobic sludge of consecutive running UASB reactor. Based on culture media of Thiobacillus denitrificans, the effects of pH, temperature, NO3 -N, NH4^＋-N, S2O3^2-, HCO3^-, C6H12O6, P, Mg^2＋ and Fe^2＋ on the denitrification by T.d.a were studied in batch experiments using sodium thiosulphate as sulfur source. The nitrate removal velocities were high at pH 6.5-8.0 and temperature 20-35 ℃. The optimal conditions were pH 7.04 and 27.40℃. T.d.a exhibited a good adaptation when the concentration of NO3-N was below 416mg/L. At 554mg/L or above, however, the nitrate removal was markedly inhibited. The denitrification needed NH4^＋-N and its limiting concentration was 2.62mg/L. The effect of the concentration of S2O32 on the denitrificaion depended on different sulfur to nitrogen ratios. When NO3 was enough, the NO3^ -N removal efficiency was in similar direct proportion to the S2O3^2- added[0]. Using HCO3^- as sole carbon source, its limiting concentration was 29.05mg/L. The presence of glucose up to 2000mg/L didn＇t significantly affect the nitrate removal efficiency. The limiting concentrations of P and Mg^2＋ were 0.034mg/L and 0.059mg/L respectively. The limiting concentration of Fe^2＋ was below 0.058mg/L.