中文摘要：

目的：观察肠缺血/再灌注大鼠血浆乙酰胆碱（ACh）含量变化及其与内皮细胞活化相关黏附分子和致炎因子含量变化的关系,初步探讨电针刺激足三里穴的抗炎机制.方法：40只成年雄性Wistar大鼠随机分为假手术组（S组）、肠缺血/再灌注组（IR组）、肠缺血/再灌注＋电针足三里穴组（IRE组）和肠缺血/再灌注＋电针非经非穴组（IRSE）.采用肠系膜上动脉夹闭45 min后松夹恢复血流的方法复制肠缺血/再灌注模型;IRE组和IRSE组在肠系膜上动脉夹闭15 min后分别电针刺激足三里穴和非经非穴,持续30 min.ELISA方法检测各组再灌注后1 h和3 h血浆ACh、E-选择素、细胞间黏附分子-1（ICAM-1）、血管内皮生长因子（VEGF） 和TNF-α含量.结果：肠缺血/再灌注1 h和3 h血浆TNF-α含量、E-选择素、ICAM-1及 VEGF含量增加,而肠缺血/再灌注3 h血浆ACh含量降低（与肠缺血/再灌注1 h比较,P〈0.05）;与IR组相比,IRE组血浆TNF-α、VEGF、E-选择素和ICAM-1含量呈不同程度的减少,血浆ACh含量回升.IRSE组在再灌注3 h时,血浆ICAM-1含量也比IR组再灌注3 h减少,但不能使血浆ACh含量回升.结论：肠缺血/再灌注时内皮细胞活化及致炎活性增强与机体胆碱能活性降低有关;电针刺激足三里穴可增加胆碱能活性,抑制致炎因子和内皮细胞黏附分子的表达和释放.

英文摘要：

Objective：To observe changes in acetylcholine（ACh）content in the plasma of rats with intestinal is-chemia/reperfusion and its relation to change in contents of endothelial adhesion molecules and proinflammatory mediators, and to explore the antiqnflammatory mechanism of electroacupuncture at Zusanli point （足三里穴）. Methods： Forty adult male Wistar rats were randomly divided into four groups： sham intestinal isehemia/reperfu- sion（S）,intestinal ischemia /reperfusion（IR）, intestinal ischemia/reperfusion plus electroacupuncture at Zusanli point（IRE）, and intestinal ischemia/reperfusion plus sham electroacupuncture （IRSE）groups. The intestinal is-chemia/reperfusion was replicated by occluding the superior mesenteric artery （SMAO）for 45 minutes, followed by 1 hour and 3 hours of intestinal reperfusion （IRlh and IR3h）. Rats in IRE and IRSE groups were subjected to electroacupuncture at Zusanli point or sham electroacupuncture for 30 minutes after SMAO for 15 minutes. En- zyme linked immunoadsorbent assay （ELISA）method was adopted to determine the contents of plasma ACh, E-se-lectin, intercellular adhesion molecule-1 （ICAM-1）, vascular endothelial growth factor（VEGF）and tumor necrosis factor-α（TNF-α）in rats with intestinal reperfusion for 1 hour and 3 hours following 45 minutes of ischemia. Re-suits： The increase in the plasma contents of TNF-α, E-selectin, ICAM-1 and VEGF was delayed in IRlh and IR3h rats, and plasma ACh content was reduced in IR3h rats（P〈0.05, vs, IRlh）. IRE rats showed decreased plasma TNF-α,VEGF and soluble adhesion molecules contents, and enhanced plasma ACh content in comparison with IR rats. Compared to the IR group, plasma TNF-α and ICAM-1 contents also decreased in IRSE rats after reperfusion for 3 hours, and plasma ACh content also decreased. Conclusions： These findings indicate that activa- tion of endothelial cell and enhancement of inflammatory reaction after ischemia and reperfusion of intestine may be related to de