本试验旨在研究饲粮添加不同水平的β-胡萝卜素对肉牛生产性能、抗氧化功能、血液生理指标和肉品质的影响。选用体况良好、平均体重为(381.00±26.01) kg的西门塔尔杂交阉牛120头,随机分为4组,每组30头,对照组饲喂基础饲粮,试验组饲喂在基础饲粮中分别添加600、1200和1800 mg/d β-胡萝卜素的试验饲粮。预试期10 d,正试期90 d。结果表明:1)饲粮添加不同水平的β-胡萝卜素对末重、平均日增重和干物质采食量的影响均不显著(P>0.05)。2)与对照组相比,1200 mg/d β-胡萝卜素试验组显著提高了血清谷胱甘肽( GSH)含量和总超氧化物歧化酶(T-SOD)活性(P<0.05),而血清丙二醛(MDA)含量则显著降低(P<0.05);1800 mg/d β-胡萝卜素试验组显著提高了血清总抗氧化能力( T-AOC)(P<0.05);随着β-胡萝卜素添加水平的升高,血清T-AOC、GSH含量、T-SOD活性和MDA含量均呈显著的线性和二次改变(P<0.05)。3)与对照组相比,600 mg/d β-胡萝卜素试验组显著降低了血液中间细胞( MID)计数和百分比(P<0.05);1800 mg/d β-胡萝卜素试验组血液中性粒细胞( GRAN)百分比显著升高( P<0.05);随着饲粮β-胡萝卜素添加水平的提高,血液MID计数和百分比呈显著的线性和二次变化(P<0.05),血液GRAN百分比以及白细胞(WBC)和淋巴细胞(LYM)计数均呈显著的线性变化( P<0.05)。4)与对照组相比,1200 mg/d β-胡萝卜素试验组显著降低了肉色黄度(P<0.05);随饲粮β-胡萝卜素添加水平的提高,肉色亮度有线性降低的趋势(P=0.079);不同β-胡萝卜素添加水平对肉中粗蛋白质、粗脂肪、水分、粗灰分含量以及肉色亮度和红度影响均不显著(P>0.05)。总之,肉牛饲粮添加β-胡萝卜素能够显著影响抗氧化功能、血液生理指标和肉品质,本试验条件下,?
This experiment was conducted to investigate the effects of dietary supplementation of different levels of β-carotene on performance, antioxidant function, blood physiological indices and meat quality of beef cattle.One hundred and twenty healthy Simmental crossbred bullocks with similar weight of (381.00 ±26.01) kg were randomly divided into four groups with 30 bullocks in each group.Beef cattle in control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 600, 1 200 and 1 800 mg /d β-carotene, respectively, during 10 days of pre-experiment and 90 days of formal experiment. The results showed as follows: 1) no significant differences in final weight, average daily gain and dry matter intake were found in beef cattle received different dietary supplemental levels of β-carotene (P〈0.05).2) Compared with control group, serum glutathione (GSH) content and total superoxide dismutase (T-SOD) activity in 1 200 mg /d β-carotene experimental group were significantly increased (P〈0.05), however, serum malondialdehyde (MDA) content was significantly decreased (P 〈0.05); serum total antioxidant capacity (T-AOC) in 1 800 mg /d β-carotene experimental group was significantly increased (P <0.05).Significant linear and quadratic changes were found in serum T-AOC, GSH content, T-SOD activity and MDA content with the increase of dietary β-carotene supplemental level (P〈0.05).3) Compared with control group, blood middle cell (MID) count and percentage in 600 mg /d β-carotene experimental group were significantly decreased (P〈;0.05), while blood neutrophile granulocyte (GRAN) percentage was significantly increased in 1 800 mg /d β-carotene experimental group (P〈0.05); with the increase of dietary β-carotene supplemental level, significant linear and quadratic changes were found in blood MID count and percentage (P〈0.05), and significant linear changes were found in blood GRAN percenta