目的:研究江西虫草菌丝体的化学成分。方法:以深层发酵的方法制备江西虫草菌丝体;运用硅胶柱、凝胶柱、制备薄层层析等多种色谱方法进行分离纯化;利用常规理化分析以及1H-NMR、13C-NMR和MS等波谱技术鉴定其化学结构。结果:从江西虫草菌丝体的甲醇提取物中石油醚和乙酸乙酯两个萃取部位,分离得到9个化合物,分别鉴定为:尿嘧啶(Ⅰ)、腺嘌呤(Ⅱ)、腺嘌呤核糖核苷(Ⅲ)、尿嘧啶核苷(Ⅳ)、3′-甲氧基尿嘧啶核苷(Ⅴ)、丁二酸(Ⅵ)、烟酸(Ⅶ)、十八烷酸-α-单甘油酯(Ⅷ)、二十四烷酸(Ⅸ)。结论:首次对江西虫草菌丝体进行了较系统的分离纯化,从中获得9个化合物,其中化合物Ⅴ、Ⅶ、Ⅷ、Ⅸ为首次从虫草属真菌中分离得到。
Objective:In an effort to investigate the chemical constitutes of submerged cultivation mycelium of Cordyceps jiangxiensis anamorph.Methods:The mycelia of C.jiangxiensis anamorph produced by submerged cultivation technique were separated and purified using various chromatographic techniques such as repeated liquid column chromatography,preparative thin layer chromatography etc.,and then the chemical structures of compounds obtained were elucidated on the basis of physicochemical analysis and spectroscopic techniques such as 1H-NMR,13C-NMR and MS.Results:Nine compounds were obtained from the petroleum ether and ethyl acetate extractive fractions of mycelial methanol extract,and were identified as follows: uracil(Ⅰ),adenine(Ⅱ),adenosine(Ⅲ),uridine(Ⅳ),3′-methoxyuridine(Ⅴ),succinic acid(Ⅵ),nicotinic acid(Ⅶ),1-monostearin(Ⅷ),tetracosanoic acid(Ⅸ).Conclusion:Chemical constitutes of C.jiangxiensis anamorph are systematically separated for the first time,and compounds Ⅴ,Ⅶ,Ⅷ,and Ⅸ obtained are separated from Cordyceps species firstly.