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中文摘要：
目的观察体外培养的大脑细胞Ⅱ、Ⅲ型脱碘酶（D2、D3）mRNA在甲状腺激素的作用下其表达量的变化。方法取胎龄15～18d Wistar大鼠脑组织．机械分散后接种细胞，培养12d后分成4组．按组分别加入100nmoL／L T3、T4、地塞米松，对照组不做处理。在培养的不同时间收集细胞提取RNA，半定量RT-PCR方法分析D2-mRNA和D3-mRNA的变化。结果T3作用1、2、4、6h后，D2-mRNA表达量比对照组降低9％～11％（t值分别为13．94、5．76、15．01、12．30，P〈0．05）。D3-mRNA表达量在作用4、6h后有明显增加，电泳条带从无到较为清晰。T4作用后，D2-mRNA、D3-mRNA的表达量在各时间点未见明显的变化；地塞米松作用1、2、4、6h后，D2-mRNA表达量比对照组上调18％～26％（t值分别为14．29、10．89、15．55、10.38．P〈0．05）。结论大鼠脑细胞在体外培养时，T3对D2-mRNA的表达有抑制的作用，对D3-mRNA的表达有促进作用；T4对D2-mRNA、D3-mRNA的表达无明显影响；地塞米松有促进D2-mRNA表达的作用．但不影响D3-mRNA的表达。
英文摘要：
Objective To observe the effect of thyroid hormones on expression of type Ⅱ and Ⅲ deiodinases in brain cells in vitro. Methods Fetal cerebral hemispheres of Wistar rats at 15 - 18 clays of gestation were dissected, dissociated, and planted. After cultured for 12 clays,they were divided into 4 groups, those added with 100 nmoL/L of T3,T4 and dexamethasone respectively were experimental groups. At different times brain cells of each group were collected for semi-quantity RT-PCR and were analyzed of the variation of D2-mRNA and D3- mRNA. Results Expression of D2-mRNA in brain cells in 100 nmoL/L T3 group was significantly decreased by 9% - 11%（t = 13.94,5.76,15.01,12.30, P 〈 0.05） after 1 hour, 2 hours,4 hours and 6 hours. The expression of D3- mRNA markedly increased 4 hours and 6 hours after T3 was added ,and electrophoresis bands became to appear clearly. No change of D2-mRNA and D3-mRNA was observed in 100 nmoi/L T4 groups. Dexamethasone stimulated D2- mRNA in cultured brain cells at 1 hour, 2 hours, 4 hours and 6 hours. It increased by 18% - 26% （t = 14.29,10.89,15.55,10.38,P 〈 0.05）. Conclusions T3 inhibits the expression of D2-mRNA, but enhances that of D3-mRNA in the cultured brain cells. T4 has no obvious change on transcription of D2-mRNA and D3-mRNA. Dexamethasone stimulates the expression of D2-mRNA and has no effect on D3-mRNA.
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