中文摘要：

选择壳聚糖水溶液作为唯一反应物,利用简单方便的微波原位法制备碳点/壳聚糖荧光复合物,该复合物无需经过产物分离提纯等步骤就能够直接进行后续应用.测试结果表明,碳点/壳聚糖复合物溶液呈黄棕色,透明均一,在365 nm紫外光下显示出明显的蓝色荧光.此外,碳点/壳聚糖复合物中的碳点分散良好,尺寸比较均一.重要的是,该碳点/壳聚糖复合物具有新的综合性能特点,即不仅被赋予了碳点的多色荧光性能,而且还保留了壳聚糖的成膜性能和p H刺激响应性能,可以十分方便地直接进行多种应用,包括利用该复合物制备荧光复合膜和复合球,以及采用电沉积方法制备具有特定形状的荧光涂层,还可以直接应用于细胞成像.因此,这种微波原位法制备的碳点/壳聚糖复合物在荧光材料、荧光涂层、生物成像和生物标记等领域具有潜在的应用价值.

英文摘要：

On the basis of microwave treatment of chitosan solution,a convenient in situ method is established to form fluorescent carbon dots / chitosan composites which can be directly used in subsequent applications. This method has many benefits including prompt operation,simple equipment,simplified post-treatment,＂green ＂process and low cost,and it allows a convenient way to gain carbon dots / chitosan nanocomposites that can be directly used for wide applications. The analysis results indicate that the carbon dots / chitosan composite solution is transparent and homogeneous. In addition,the carbon dots / chitosan composite solution displays brown color under visible light,while presents clear blue fluorescence under 365 nm UV light. Transmission electron microscopy test exhibits that carbon dots disperse well in the carbon dots / chitosan composite,and the average size of carbon dots is about 7. 0 nm. Specially,the obtained carbon dots / chitosan composite not only has attractive properties（ e. g.,multicolor fluorescence） that belong to carbon dots,but also retains the favorable properties（ e. g.,p H-responsive properties） of chitosan. Based on these combined properties,it is convenient to employ the carbon dots / chitosan composite to construct fluorescent films by casting method,to prepare fluorescent beads by a convenient dropping procedure,as well as to generate fluorescent coatings on electrodes by electrodeposition technique. Furthermore,the carbon dots / chitosan composite can be directly applied to cell imaging. Thus,this carbon dots / chitosan composite constructed by in situ method has potential applications in fluorescent materials,electrodeposited coatings,drug carriers,bioimaging and biolabeling.