中文摘要：

为提高小球藻（Chlorella sp．）的生物量，须对f／2配方培养基进行响应面优化。首先须确定小球藻培养基的最佳pH值和盐度。在此基础上，利用Plackett—Burman设计方案筛选出影响小球藻生长的3个主要因素分别为NaHCO3、KNO3和维生素B12，然后通过Box—Behnken设计试验确定这3个主要因素的最佳质量浓度参数。结果表明，当培养基组成为：NaHCO3 0．93g／L、MgSO4 0．40g／L、KNO3 0．46g／L、K2HPO4 0．020g／L、维生素B1 0．60mg／L、维生素B12 1．8μg/L、生物素2．0μg／L时，小球藻经实验室培养72h后的生物量达到4．5×10^7个／mL，较优化前提高了32．5％。

英文摘要：

In order to increase the biomass yield of Chlorella sp. , the culture conditions of f/2 medium were optimized by the response surface method. It was found that pH 5 and salinity 40 of the medium were suitable for the algal growth. Three important substrates in the culture medium screened via Plackett - Burman were sodium bicarbonate, potassium nitrated and Vitamin B12, and their concentrations were further optimized by Box-Behnken. The optimized culture conditions were as follows ： 0. 93 g/L of sodium bicarbonate, 0. 40 g/L of magnesium sulphate, 0. 46 g/L of potassium nitrate, 0. 020 g/L of potassium phosphate dibasic, 0. 60 mg/L of Vitamin Bl , 1.8 μg/L of Vitamin B12, and 2. 0 μg/L of biotin. After optimization, the cell density of the algae cultured for 72 hours was 4. 5 × 10^7cell/mL, which was 32. 5% more than that in the original culture medium.