中文摘要：

本实验建立了一种灵敏且专属的HPLC-DAD测定家兔关节液中头孢唑林钠的方法。用乙腈沉淀关节液中的蛋白质,安替比林作为内标。样品在戴安U3000高效液相色谱仪上操作,并用菲罗门Luna C18（150 mm×4.60 mm,5μm,100 A）柱分离,流动相为0.1%甲酸水和乙腈,流速为1 mL/min,检测波长为272 nm,柱温为25 oC。在1.0–100.0μg/mL范围内,头孢唑林钠和内标安替比林的峰面积比值与浓度线性关系良好（r 2=0.9999）,最低检测限为（LOD,S/N=3）为0.07μg/mL,最低定量限（LOD,S/N=10）为0.22μg/mL。头孢唑林钠回收率（低、中、高浓度）分别为124.6%、117.8%、100.6%（RSD%=1.9%,4.0%,1.1%,n=5）。日内和日间精密度在0.5%–2.7%。本实验建立的家兔关节液中头孢唑林钠HPLC-DAD分析方法简便、灵敏且可靠。此方法可以应用于家兔关节液中头孢唑林钠的测定。

英文摘要：

A sensitive and specific high performance liquid chromatography method was established for measuring cefazolin sodium level in rabbit synovial fluid. Acetonitrile was used to precipitate proteins and antipyrine was used as internal standard. Samples were analyzed on a Dionex Ultimate U3000 HPLC system equipped with Phenomenex Luna C18 column （150 mmx4.60 mm, 5 Bm, 100 A）. The mobile phase consisted of acetonitrile and 0.1% formic acid in water. The flow rate was 1 mL/min. The detection wavelength was set at 272 nm. The column temperature was maintained at 25 ℃. The calibration curve was linear in the range of 1.0-100.0 μg/mL （r2 = 0.9999）. The limit of detection （LOD, S/N - 3） was 0.07 μg/mL. The limit of quantitation （LOD, S/N = 10） was 0.22 pg/mL. The recovery of cefazolin sodium （low, medium and high） was 124.6%, 117.8%, and 100.6% （RSD% - 1.9%, 4.0%, 1.1%, n = 5）, respectively. The intra-day and inter-day precision values were in the range of 0.5%-2.7%. The method was simple, sensitive and reliable. It can be used for the quantitative determination of cefazolin sodium in rabbit synovial fluid.