中文摘要：

以单分散交联聚甲基丙烯酸环氧丙酯（PGMA/EDMA）微球作为基质载体,溶菌酶为模板分子,β-环糊精和丙烯酰胺为复合单体,N,N′-亚甲基双丙烯酰胺为交联剂,采用表面印迹法制备了溶菌酶分子印迹聚合物;以此聚合物为固定相进行前沿色谱分析和识别性能研究.结果表明,该分子印迹聚合物对模板分子溶菌酶具有特异识别能力,基于此可以分别实现溶菌酶与牛血清白蛋白、卵白蛋白及肌红蛋白的色谱分离.

英文摘要：

Lysozyme-based molecularly imprinted polymers （Lys-MIPs） were prepared with POMA/EDMA beads as a carrier material, lysozyme as the template molecule, β-cyclodextrin and acrylamide as the double functional monomers, N,N＇-methylene bisacrylamide as the crosslinker. The prepared Lys-MIPs were used as a new HPLC packing material, based on which the related study on its recognition was conducted through the frontier chromatography. The results showed that Lys-MIPs had specific recognition for lysozyme, which helped achieve the separation between lysozyme and three individual competitive proteins, including bovine serum albumin （BSA）, ovalbumin（OB） and myoglobin （Myo）.