中文摘要：

目的评价壳寡糖作为基因载体的理化性质及转染293T细胞的活性。方法选用相对分子质量小的壳寡糖，使用噻唑蓝（MTT）法检测不同浓度壳寡糖的细胞毒性，琼脂糖凝胶电泳检测壳寡糖结合及保护质粒DNA的作用，荧光显微镜观测转染结果。结果高浓度壳寡糖第2天时具有较低毒性，第4天时细胞存活率高于不做处理的对照细胞；质量比〉20倍时，壳寡糖几乎完全结合DNA，并且具有一定保护的作用；转染293T细胞后，荧光显微镜能够观测到表达的绿色荧光蛋白。结论壳寡糖可以作为基因载体转染293T细胞。

英文摘要：

Objective To evaluate the physicochemical property of chito-oligosaceharides and the activity of the transfected 293T cells. Methods Chito-oligosaccharides of small relative molecular mass was used in our study. The cytotoxicity of different chito-oligosaccharides was measured by MTT. The capability of chito-oligosaccharides binding and protecting plasmid DNA was detected with agarose gel eleetrophoresis. The result of transfection was observed under fluorescence microscope. Results On the second day, high concentration of chito-oligosaccharides demonstrated little toxicity. On the fourth day, the cell survival rate of all experimental groups was higher than that of the control group. Chito-oligosaccharides exhibited complete binding with all DNA and effective protection when the mass ratio was over 20. Green fluorescence was found after transfection. Conclusion Chito-oligosaccharides was used as gene verors to transfect 293T cells.