中文摘要：

目的探讨S-腺苷酰-L-甲硫氨酸（S-adenosyl-L-methionine,SAMe）对肝癌细胞HepG2增殖、迁移及癌基因C-myc表达的影响,为深入研究SAMe在肝癌治疗中的作用奠定基础。方法体外培养HepG2细胞,MTT法检测不同浓度SAMe作用不同时间对HepG2细胞增殖的影响;细胞划痕法检测最适浓度SAMe对HepG2细胞迁移能力的影响;RT-PCR、免疫细胞化学法及Westernblot法检测SAMe对HepG2细胞癌基因C-myc转录、蛋白定位及表达的影响。结果终浓度为15.0μmol/L的SAMe处理细胞5d,细胞抑制率达54.6%,且抑制作用呈时间与剂量依赖性;经15.0μmol/LSAMe处理的细胞迁移能力明显下降,愈合速率为对照组的76.78%;细胞中癌基因C-myc的转录和蛋白表达均明显下降,蛋白定位无明显变化。结论 SAMe可通过降低C-myc的表达,抑制HepG2细胞的增殖和迁移,为肝癌治疗性药物的研究提供了一个新的方向。

英文摘要：

Objective To investigate the effect of S-adenosyl-L-methionine（SAMe）on the proliferation and migration of human liver cancer HepG2 cells as well as the expression of cancer gene C-myc, and lay a foundation of further study on the role of SAMe in therapy of liver cancer. Methods HepG2 cells were cultured in vitro and treated with SAMe at various concentrations for various days, and the effect on cell proliferation was determined by MTT method. The effect of SAMe at optimal concentration on the migration of HepG2 cells was determined by cell streak. The effects of SAMe on the transcription of C-myc gene as well as location and expression of protein in HepG2 cells were determined by RT-PCR, immunocytochemical method and Western blot respectively. Results The inhibition rate of HepG2 cells after treatment with SAMe at a final concentration of 15. 0 μmol / L for 5 d reached 54. 6%, and the inhibitory effect was time- and dose-dependent. The migration ability of HepG2 cells after treatment with 15. 0 μmol / L SAMe decreased significantly, with a speed for healing of streak equivalent to 76. 78% of that in control group. Both the transcription level of C-myc gene and expression of C-myc protein in HepG2 cells treated with SAMe decreased significantly, while the location of protein showed no significant change. Conclusion SAMe inhibited the proliferation and migration of HepG2 cells by decreasing the expression of C-myc gene, which provided a new pathway for development of drugs for liver cancer.