中文摘要：

目的：探讨伤寒沙门菌调节因子UhpA的功能。方法：用载体pBAD介导回补uhpA至伤寒沙门菌uhpA基因缺陷变异株,用qRT-PCR观察cysM和treB基因表达,用表达载体pET-22b原核表达UhpA蛋白,用凝胶阻滞试验观察UhpA蛋白与cysM和treB启动子区域DNA片段的结合。结果：成功构建pBADuhpA重组质粒,在uhpA缺陷变异株中回补uhpA基因后,cysM和treB的表达明显恢复,但UhpA-H is6蛋白与cysM和treB的启动子区域DNA片段无明显结合。结论：伤寒沙门菌UhpA在高渗应激条件下能促进硫代谢及海藻糖代谢相关基因表达,且可能为间接作用。

英文摘要：

Objective： To explore the function of the regulator UhpA in Salmonella enterica serovar Typhi.Methods： A recombinant plasmid pBADuhpA containing the uhpA gene with its own promoter was transferred into the uhpA deleted mutant of S.entericar serovar Typhi.qRT-PCR was performed to observe the expression of cysM and treB.UhpA protein was expressed by a recombinant plasmid pET-22buhpA in E.coli.The gel shift assays was performed to explore the combination between UhpA and the promoter region of cysM and treB.Results： Recombinant plasmid pBADuhpA was successfully constructed.After complementing uhpA gene in the uhpA deleted mutant,the expression of cysM and treB was obviously restored.Gel shift assays showed that UhpA protein could not bind the promote region of cysM and treB.Conclusion： At up-shift high osmotic treatment,the UhpA may promote the expression of genes involved in the metabolism of sulfur and trehalose indirectly.