中文摘要：

【目的】分离授粉早期玉米花丝的总蛋白质,为从蛋白质组角度揭示玉米花粉与花丝早期的相互作用奠定基础。【方法】以玉米（ZeamaysL.）自交系Ga25为试材,采用三氯乙酸-丙酮法提取总蛋白质,运用双向电泳、质谱分析与检索技术,比较自交授粉后1h和2h的花丝蛋白质组的差异。【结果】与授粉1h的蛋白质组相比,在授粉2h后的蛋白质组中出现28个差异蛋白点,其中,6个蛋白质点特异表达,19个蛋白质点上调表达,3个蛋白质点下调表达;通过MALDI-TOF-MS质谱测序和MASCOT序列分析,注释了23个蛋白质点,其余5个为未知蛋白;功能分类表明,差异蛋白质分别参与细胞壁合成（21.4%）、防御/抗胁迫（17.9%）、细胞组织、蛋白命运、蛋白合成、转录等细胞过程。【结论】在玉米花粉与花丝早期的相互作用过程中蛋白质组有显著的变化,与授粉后1h相比,授粉后2h的蛋白质组中大部分差异蛋白呈上调趋势,并有特异蛋白质出现;分泌型过氧化物酶、膨胀素、果胶甲基化酶抑制蛋白、谷胱甘肽转移酶与未知蛋白4可能与玉米花粉和花丝的早期互作密切相关。

英文摘要：

【Objective】 The purpose of this project was to study the early pollen-silk interaction in maize through proteomic analysis.【Method】 Inbred line Ga25 was pollinated and total silk proteins were extracted after 1 h and 2 h pollination.Total proteins were extracted by TCA/acetone,separated by two-dimensional gel electrophoresis （2-DE） and analysed through MALDI-TOF-MS mass spectrometry.【Result】 Compared with the pollen/silk proteome after 1 h pollination,the proteome after 2 h pollination contained 28 differentially expressed proteins,including 6 specifically expressed ones,19 up-regulated and 3 down-regulated ones.And 23 of them were identified homologous to known proteins in various databases by MALDI-TOF-MS and MASCOT analysis.The predicted functions of the 23 proteins were related to cell wall biosynthesis （21.4%）,defense/stress responses （17.9%）,cellular organization,protein fate,protein synthesis and transcription.【Conclusion】 The pollen/silk proteome profiles were different between the 1 h and 2 h pollination intervals.The majority of differentially expressed proteins were up-regulated;down-regulated and speciafically expressed proteins were also found in the 2 h pollination proteome profile.Secretory peroxidase,expansin,pectin methylesterase inhibitor,glutathione S-transferase and unknown protein 4 may play important roles in early pollen-silk interactions in maize.