中文摘要：

以野生平榛（C.heterophylla Fisch.）雌花序为供试材料,研究榛子木质化花柱软化的方法.结果表明：（1）1.0 mol/L Na OH溶液浸泡并煮沸比相同处理下蒸馏水的软化效果好;（2）1.0 mol/L Na OH溶液浸泡材料并立即水煮软化20 min后的软化效果优于其他处理时间;（3）苯胺蓝染色环境的缓冲液最佳p H范围为5.7至7.1.此改进方法可以在一天内即可完成榛子花粉管形态的观察,较传统软化方法简单、快捷.

英文摘要：

Pistilate flower of wild hazelnut （ C.heterophylla Fisch.） was used as study materials to improve style softening method.The results showed that：（1） 1.0 mol/L boiling NaOH solution soaking was more beneficial for softening of style compared with boiling distilled water;（2） the optimal treatment time was 20 min in 1.0 mol/L boiling NaOH solution;（3） The suitable buffer pH of aniline blue solution ranged from 5.7 to 7.1.After improvement,morphology of pollen tubes of hazelnut could be observed clearly within one day .Thus,this method is more simple and efficient than the traditional one .