中文摘要：

目的：观察家蝇蛹血淋巴及其提取物对小鼠脾淋巴细胞增殖的影响。方法：以不同浓度血淋巴及其提取物与脾淋巴细胞、T细胞和B细胞共孵育72小时,采用MTT法测定淋巴细胞的吸光度值,观察它们对淋巴细胞增殖的促进作用,采用免疫印迹法研究提取物对B淋巴细胞增殖的信号转导通路。结果：与对照组相比,血淋巴冻干粉、分子量在50kD以上的冻干粉及其提取物对混合淋巴细胞和B细胞增殖有显著促进作用,然而对于T细胞的增殖却没有明显的促进作用。MTT实验和免疫印迹结果表明血淋巴提取物激活了B淋巴细胞的ERK通路,促进了B淋巴细胞的增殖。结论：家蝇蛹血淋巴及其提取物具有免疫调节作用,为天然免疫增强剂的开发提供了一定的参考依据。

英文摘要：

Objective：To observe the function of Musca domestica pupae hemolymph on mouse splenocytes proliferation and methods for its extraction. Methods：Different concentrations of Musca domestica hemolymph after extraction were cultured with splenocytes, T lymphocytes and B lymphocytes for 72 h, and the effect of promoting proliferation was detected by the method of MTF test by optical density. Western blot was used to examine the signal transduction pathway involved in B lymphocyte proliferation, which was stimulated by the extracs. Results： The hemolyph and its extact with the molecular mass above 50 kD significantly promoted the proliferation of mixed splenocytes and B lymphocytes than those of the control group, nevertheless, the proliferation effect of T lymphocytes was not significant. Result of MTT trial and Western blot showed that ERK signaling pathway in B lymphocytes proliferation was activated by hemolymph extract. Conclusion： These results show that the Musca domestica pupae hemolymph and its extract possess immunomodulation effect, which provide certain reference basis for the development of natural immunopotentiator.