[目的]为稀土农用拓展及环境污染防治提供策略。[方法]采用水培法培养大豆,待第3枚三出复叶展开后进行La和UV-B辐射处理。以前期试验确定的最适浓度LaCl3(20mg/L)溶液均匀喷洒大豆叶片,48 h后,放置于特定剂量的紫外灯下进行胁迫处理。UV-B设2个处理:T1(0.15 W/m^2)、T2(0.45 W/m^2),每天处理6 h(10:00-16:00),连续处理5 d,每天同一时间取同向、同叶位大豆幼苗叶片测定叶绿素(Ch1)、丙二醛(MDA)、H_2O_2膜脂脂肪酸、吲哚乙酸(IAA)和脱落酸(ABA)的含量。[结果]UV-B(T_1:0.15 W/m~2和T_2:0.45 W/m~2)辐射下,大豆幼苗叶绿素(Ch1)和吲哚乙酸(IAA)含量在胁迫期(1~5 d)逐渐下降,在恢复期(6-11 d)上升;丙二醛(MDA)和脱落酸(ABA)含量先增(1~5 d)后减(6~11 d)。20 mg/L La(Ⅲ)减缓了上述指标在胁迫期的下降/上升趋势,加快了恢复期的上升/下降速度。[结论]La(Ⅲ)对大豆幼苗膜脂过氧化和内源激素的调控能提高叶绿素和IAA含量,改善活性氧的新陈代谢,抑制膜脂过氧化作用,减少ABA积累,且对低剂量紫外辐射(T_1)的防护效果优于高剂量紫外辐射(T_2)。
[ Objective] The aim was to provide strategies for development of rare earth and control of environmental pollution. [ Method] Responses of membrane lipid peroxidation and endogenous hormones of soybean seedlings to UV-B radiation and rare earth were studied through hydroponics in laboratory. [ Result] The results showed that under irradiation of UV-B( T1-0.15 W/m^2 and T2-0.45 W/m^2), chlorophyll and indole-3-acetic acid(IAA) contents firstly decreased during the stress phase (1 -5 d) and then increased during the restoration phase (6 -9 d) while contents of malonadialdehyde(MDA) and abscisic acid(ABA) gradually increased during the imposition of UV-B radiation (1 -5 d) and subsequently decreased during recovery from UV-B stress (6 -9 d) . With adding of La (III) with the concentration of 20 mg · L^-1 , the decline/dse trend of chlorophyll, IAA, MDA and ABA contents was slowed down during the stress period while the rise/decline speed was accelerated during the recovery period. [ Conclusion] It suggests that the regulation of La ( III ) on membrane lipid peroxidation and endogenous hormones could increase chlorophyll and IAA contents, improve the metabolism of reactive oxygen species ( ROS), inhibit membrane lipid peroxidation, decrease the accumulation amount of ABA and alleviate injury of UV-B radiation to soybean seedlings. Further, the protective potential of La ( III ) was better under low UV-B radiation than under high one.