中文摘要：

以五年生人参根组织为试验材料,利用RT-PCR方法克隆人参DS基因序列;通过生物信息学技术对其核苷酸序列和氨基酸序列进行比对;运用实时荧光定量PCR技术检测DS基因在人参根、茎、叶、果各部位的表达量。结果表明：获得人参DS基因c DNA全长,其核苷酸序列长为2.385 kb,含有1个开放阅读框,编码769个氨基酸多肽。生物信息学分析显示DS编码蛋白质包含1个跨膜区域,具有3个保守结构域。人参DS编码蛋白质与西洋参的DS（AGK62449）和三七的DS（AGI19258）具有99%的序列相似性。实时荧光定量PCR显示,DS基因在人参根、茎、叶、果各部位均有表达,在叶中表达量最高,其次是在果中,在根和茎中表达量相近。

英文摘要：

Five-year-old ginseng root tissue was used for cloning DS gene by RT-PCR technology.Nucleotide and amino acid sequences were compared by bioinformatics technology. DS gene expression levels in roots,shoots,leaves and fruits of P. ginseng were detected by real-time quantitative PCR. Full-length c DNA of DS was 2. 385 kb which included an open reading frame（ ORF） encoding 769 amino acid peptide. Bioinformatic analysis showed that DS-encoding protein contained one transmembrane region and three conserved domains. Protein sequence of P. ginseng DS had the highest sequence similarity（ 99%） with P. quinquefolius DS（ AGK62449） and P. notoging DS（ AGI19258）. Real-time quantitative PCR showed that DS gene was expressed in all the detected tissues. Expression level of P. ginseng DS was the highest in leaves,followed by fruits. Roots and shoots had a similar quantitative expression.