中文摘要：

目的：建立p110δ突变失活的ApcMin/＋结直肠癌癌前病变小鼠模型，为研究p110δ在小鼠结直肠癌癌前病变中的作用提供实验模型。方法：将C57BL/6J背景的ApcMin/＋结直肠癌癌前病变小鼠与p110δ突变失活小鼠（p110δD910A/D910A）进行杂交建系，通过PCR技术鉴定子代小鼠基因型，获得p110δ突变失活的ApcMin/＋小鼠。对适龄小鼠进行肠道取材，亚甲蓝染色后，观察肠道结构，对腺瘤和微腺瘤进行统计。肠道组织进行石蜡包埋、切片，做HE染色进行进一步观察。结果：获得p110δ突变失活的Apc^Min/＋杂交鼠（ Apc^Min/＋；p110δD910A/D910A ）并得以稳定传代。 Apc^Min/＋；p110δD910A/D910A杂交小鼠的肠道组织中，腺瘤数目和体积比Apc^Min/＋结直肠癌癌前病变小鼠均有减少。结论：成功建立p110δ突变失活的Apc^Min/＋结直肠癌癌前病变小鼠模型，并得到小鼠肠道肿瘤的初步表型，为进一步研究p110δ在肠道肿瘤发生发展中的作用提供重要的工具动物。

英文摘要：

AIM：Toestablishatransgenicheterozygousmousemodelofprecancerouslesionsofcolorectal cancer with p110δmutation in the C57BL/6J background for serving the studies on colorectal cancer research mediated by p110δ.METHODS：The transgenic heterozygous mice were generated by crossing in p110δD910A/D910A mouse and ApcMin/＋mouse, and the genotype was detected by PCR .Compared with ApcMin/＋mice, transgenic heterozygous mice （ ApcMin/＋;p110δD910A/D910A）were counted, and the number and size of intestine polyps were analyzed after methylene blue staining . The intestinal tissue structure was assessed by HE staining .RESULTS：The transgenic heterozygous mouse model of pre-cancerous lesions of colorectal cancer with p 110δmutation was established .The number and size of polyps in the transgenic heterozygous mice were declined .CONCLUSION： A transgenic heterozygous mouse model of precancerous lesions of colorectal cancer with p 110δmutation was successfully established .The initial phenotype of intestinal tumors in transgenic mice was observed .This model will greatly contribute to the related research of colorectal cancer in mice .