中文摘要：

瞄准: 在 ischemia/reperfusion 损害(IRI ) 以后在老鼠大脑在神经发生上调查人参皂甙 Rd (Rd ) 的效果。

英文摘要：

Aim： To investigate the effects of ginsenoside Rd （Rd） on neurogenesis in rat brain after ischemia/reperfusion injury （IRI）. Methods： Male SD rats were subjected to transient middle cerebral artery occlusion （MCAO） followed by reperfusion. The rats were injected with Rd （1, 2.5, and 5 mg·kg^-1·d^-1, ip） from d I to d 3 after MCAO, and with BrdU （50 mg·kg^-1·d^-1, ip） from d 3 to d 6, then sacrificed on 7 d. The infarct size and neurological scores were assessed. Neurogenesis in the brains was detected by BrdU, DCX, Nestin, and GFAP immunohistochemistry staining. PC12 cells subjected to OGD/reperfusion were used as an in vitro model of brain ischemia. VEGF and BDNF levels were assessed with ELISA, and Akt and ERK phosphorylation was measured using Western blotting. Results： Rd administration dose-dependently decreased the infarct size and neurological scores in the rats with tRI. The high dose of Rd （5 mg.kg＇l〈1-1） significantly increased Akt phosphorylation in ipsilateral hemisphere, and markedly increased the number of BrdU/ DCX and Nestin/GFAP double-positive cells in ischemic area, which was partially blocked by co-administration of the PI3 kinase inhibi- tor LY294002. Treatment with Rd （25, 50, and 100 pmol/L） during reperfusion significantly increased the expression of VEGF and BDNF in PC12 cells with IRI. Furthermore, treatment with Rd dose-dependently increased the phosphorylation of Akt and ERK, and significantly decreased PC12 cell apoptosis, which were blocked by co-application of LY294002. Conclusion： Rd not only attenuates ischemia/reperfusion injury in rat brain, but also promotes neurogenesis via increasing VEGF and BDNF expression and activating the PI3K/Akt and ERK1/2 pathways.