中文摘要：

研究几种有机化合物对铁生物有效性和毒性的影响。用葡萄糖、乳糖、抗坏血酸或草酸与1.5mmol/L三价铁的混合溶液分别孵育Caco-2细胞24h后,用细胞吸收铁量作为铁有效性指标,通过3-（4,5）-二甲基-2-（2,5）-二苯基溴化四氮唑蓝（MTT）比色、乳酸脱氢酶（LDH）渗漏及细胞碱性磷酸酶（AKP）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性分析评价铁对细胞的毒性。结果表明,Fe^3＋-葡萄糖、Fe^3＋-乳糖和Fe^3＋-抗坏血酸混合物铁生物有效性较FeCl3显著（P〈0.05）提高了2.57、4.08、4.52倍,Fe^3＋-草酸使铁生物有效性降低了18.73%。Fe^3＋-抗坏血酸显著增加了细胞MTT吸收（P〈0.05）,并显著降低了LDH渗漏（P〈0.05）,而Fe^3＋-乳糖显著促进了LDH渗漏（P〈0.05）。与去离子水相比,FeCl3显著降低了细胞SOD（P〈0.01）和AKP（P〈0.01）活性,而添加有机化合物对两种酶活性有促进作用;比较而言,抗坏血酸对细胞SOD、GSH-Px和AKP活性均有显著的促进作用（P〈0.05）。

英文摘要：

In order to explore the effects of organic compounds on the bioavailability and toxicity of ferric salts,the media containing 1.5 mmol/L ferric chloride alone （as a control） or its respective mixtures with glucose,lactose,ascorbic acid and oxalic acid were used to incubate Caco-2 cells for 24 h,and after completion of the incubation,intracellular iron concentration characterizing bioavailability was measured and the cellular toxicity of ferric was assessed by MTT,lactate dehydrogenase （LDH） leakage,alkaline phosphatase （ALK） activity,superoxide dismutase （SOD） activity,glutathione peroxidase （GSH-Px） activity assays.Compared with the control medium,the cells incubated in the media containing ferric chloride/glucose,ferric chloride/lactose or ferric chloride/scorbic acid mixtures showed 2.57,4.08 and 4.52-fold increases in iron bioavailability,respectively.However,the presence of oxalic acid in the control medium resulted in a decrease of iron bioavailability of the cells by 18.73%.Compared with the control medium,the cells incubated in the medium containing ferric chloride/ascorbic acid complex exhibited a significantly higher MTT absorbance （P〈0.05） but a lower LDH leakage （P〈0.05）;in contrast,the medium containing ferric chloride/lactose mixture significantly improved LDH leakage （P〈0.05）.In addition,ferric chloride exhibited a reduction effect on the activities of SOD and ALK （P〈0.01） when compared with deionized water.However,respective additions of these organic compounds could improve the activities of SOD and ALK.On the other hand,the medium containing ferric chloride/ascorbic acid mixture had a promoting effect on the activities of SOD,ALK and GSH-Px （P〈0.05）.