中文摘要：

目的探讨合成芥酸乙醇酰胺对人肝癌细胞BEL-7404的抑制作用。方法（1）运用微型有机合成技术，以芥酸、甲醇和乙醇胺为原料、金属钠为催化剂合成芥酸乙醇酰胺，采用硅胶柱层析分离纯化，红外光谱和核磁共振谱确证结构；（2）用芥酸乙醇酰胺作用于BEL3404，用倒置显微镜镜下观察其对BEL-7404细胞的生长状况的影响，MTT法观察其抑制作用。结果合成出了芥酸乙醇酰胺并确证了其结构；芥酸乙醇酰胺浓度在50．0μg／ml以上时，能很明显的抑制BEL-404的增殖，抑制率均高于40％，IC50为101．77588μs／ml。结论采用微型合成法能够快速、方便地合成出芥酸乙醇酰胺；芥酸乙醇酰胺对BEL-7404细胞的增殖有明显的抑制作用。

英文摘要：

Objective To study the synthesis of the N-alcohol erucamide and its activity of anti-tumor in vitro. Methods （1）Through the micro-organic synthesis technology, erucic acid and methanol were coupled with ethanolamine as raw matierals, sodium as catalyst to synthesize the N-alcohol erucamide, isolation and purification a- chieved by silical gel column chromatography, and Its structure was confirmed by infrared spectra and nuclear mag- netic resonance spectrum; （2）BEL-7404 was cultured in media added to the N-ethanol erucamide. The ceils＇ s growth was observed in inverted microscope. The effect of inhibiting cancer cells was detected with MTT. Results The N-alcohol erucamide was synthesized successfully;Its strong inhibiting effect was recorded beyond 50. 0 μg/ml. The inhibiting rates were all beyond 40%, the inhibitory concentration 50% （IC50） was 101. 77588 μg/ml. Conclusion Through the microscale organic synthesis, N-alcohol erucamide can be synthesized quickly and conveniently. N-alcohol erucamide has obvious inhibiting effcet on BEL-7404 ceils in vitro.