中文摘要：

目的：从北大西洋瓜参（Cucumaria frondosa）体壁中提取纯化得到一种北大西洋瓜参岩藻聚糖硫酸酯（CF—FUC），并对其结构特征进行研究。方法：利用阴离子交换层析和凝胶过滤层析纯化出CF—FUC组分，以高效凝胶过滤色谱法测定分子质量，以离子色谱法测定硫酸根含量，以柱前衍生高效液相色谱法测定单糖组成，并通过核磁共振技术推测其精细结构。结果：CF-FUC的分子质量为360kD，硫酸根含量为（29．8±3．2）％，单糖组成为岩藻糖、半乳糖、氨基葡萄糖、氨基半乳糖物质的量比为1：0．22：0．07：0．07，岩藻糖存在两位硫酸酯化α型吡喃岩藻糖及非硫酸酯化α型吡喃岩藻糖两种形式。结论：CF-FUC结构有别于其他海参岩藻聚糖硫酸酯（SC—FUC）。

英文摘要：

In this study, fucoidan from Cucumariafrondosa body wall was purified and structurally characterized. A fraction named as CF-FUC was obtained by anion exchange chromatography and gel filtration chromatography. The fraction was analyzed for molecular weight by high-performance gel filtration chromatography, sulfate content by ion chromatography, monosaccharide composition by pre-column derivitization high-performance liquid chromatography, and fine structural characteristics by nuclear magnetic resonance. The molecular weight of CF-FUC was 360 kD with a sulfate content of （29.8 ± 3.2）%, and its monosaccharide composition included fucose, galactose, glucosamine and galactosamine at a ratio of 1：0.22：0.07：0.07. Moreover, NMR analysis revealed that the fucoidan was mainly composed of a-L-pFuc-2（OSO3） and α-L-pFuc. In conclusion, the structure of Cucumariafrondosa fucoidan is novel and different from that reported previously for sea cucumber fucoidan.