中文摘要：

利用抑制性消减杂交技术分离了板栗正常花序与芽变花序之间表达的差异cDNA片段。以芽变板栗雄花序cDNA为试验方（tester），以对照板栗雄花序cDNA为驱动方（driver），构建了一个板栗芽变花序消减文库。菌落PCR显示插入片段主要在100～1000bp，文库质量良好。通过对文库阳性克隆随机测序共得到了30个有效EST序列。利用Blast同源性比较．其中一些EST可能与板栗雄花序发育中变短的生物学形态形成密切相关，为后续板栗短雄花序芽变相关基因的研究奠定了基础。

英文摘要：

In order to uncover the mechanism of sport-related genes in short-catkin chestnut,the suppression subtractive hybridization technique was used. One differential expressing cDNA library was constructed with the cDNAs of formal catkin as driver and the cDNAs of mutant catkin as tester. PCR results suggested that the insertion segments were between 100 to 500 bp and the library was suitable for the following work. Clones were randomly selected and sequenced, and 30 unique ESTs were obtained. The ESTs were annotated by similarity to orthologs and paralogs detected with Blastn and Blastx. Some of the ESTs might be related closely with biological development of the sport in short-catkin chestnut,which lays a foundation for the further study on mutant related genes of short-catkin chestnut.