采用氧化还原法制备氧化石墨烯(GO),通过原位插层聚合法制备出GO与导电聚苯胺(PANI)复合层状纳米材料。采用旋涂法将GO-PANI复合材料旋涂在自组装有十八烷三甲氧基硅烷的硅片上,对核糖核酸(DNA)在复合材料表面上的固定进行电化学性能测试。结果表明,DNA能够很好地固定在GO-PANI复合材料表面上,对将来开发出易于制备的电化学性能优良的DNA芯片提供了实验基础。
Graphene oxide(GO)was prepared by method of oxidation reduction process, the nanocomposite of graphene oxide-polyaniline (GO-PANI) via the intercalated polymerization method in situ was constructed. In order to characterize properties of the nanocomposite easily, GO-PANI was spin-coated onto the silicon substrate self-assembled by trimethoxyoctadecylsilane. The electrochemical properties of GO-PANI nanocomposite before and after DNA immobilization/hybridization were detected. It suggests that probe DNA could be immobilized onto the surface of GO-PANI nanocomposite due to the electric static interaction between GO-PANI and DNA in the buffer solution. It could supply the facible method to prepare the electrochemical biosensor with good properties.