采用刚果红羧甲基纤维素钠培养基法、滤纸条降解法和胞外酶活性测定法从长白山原始森林的腐烂枯木和落叶腐质土中筛选一株高产纤维素酶的真菌,结合菌落形态学和分子生物学方法鉴定该菌株为产黄青霉,命名为CBS-1.滤纸崩解实验说明在24h该菌对滤纸就具有明显的崩解能力,6d后滤纸的失重率达到24.1%;以稻草粉为底物发酵120h后,其胞外纤维素酶活性达到33.6mkat/L,其活性高于已经报道的菌株,是一株具有研究开发潜力的纤维素酶生产菌株.
One fungi with high efficient cellulose degrading ability was isolated from the decomposed dead wood and defoliation soil of Changbai mountain primary forest, using hydrolysis spot diameter measurement method of Congo red and CMC-Na, degradation analysis method of filter paper sheet and measurement method of enzymatic activity of cellulase. The strain was identified as PeniciUium Chrysogenum with conventional morphology method as well as modem molecular biology method, named CBS-1. Filter paper sheet degradation analysis showed that strain CBS-1 had high level capacity of decomposition in 24 hours and the loss ratio of the ilter paper sheet reached 24. 1% in 6 days, and the extracellular cellulase activity of strain CBS-1 was 33.6 mkat/L when the powers of rice straw was used as the sustrates of the enzymes, which is higher than that reported in the previous reference. Therefore, strain CBS-1 was a cellulase-producing strain with broad development potential.