SSR引物序列常用于近缘物种的遗传学研究,但存在很大的盲目性和分析结果的不可预见性。本研究采用我国9种披碱草属植物,利用不同来源微卫星引物进行遗传学分析,结果显示,披碱草属的6个和小麦的5个微卫星引物在我国9种披碱草属植物具有稳定的扩增效果和较好的遗传学分析功能;相对而言,位点ECGA22、EC—GA114、EAGAS1和WMS43遗传信息含量较高,对构建披碱草属植物高密度的微卫星种(品种)指纹是很好的选择;披碱草属植物的引物所检测到的等位基因数目与测序开发引物时预期的等位基因数目相差较少、遗传多样性指数较高和更接近研究材料的基因流,预示着亲缘关系更近的引物遗传分析的效率更高。
Microsatellite markers from related species are commonly used in plant genetics, but much unpredictability exists. Different sources of microsatellite markers were amplified in nine Elymus species in China. Results indicated that six Elymus and five Triticurn aestivum markers had stable PCR amplification and gave better genetic analytical analysis. Loci ECGA22, ECGA114, EAGA51 and WMS43 were highly informative and helpful in construction of high-resolution genetic fingerprints of Elymus taxa from China. There were few differences in allele numbers between this research and that in former publications using higher genetic indexes and similar gene flow to the materials used in this study. This suggests that more homologous primers are even more efficient.