中文摘要：

为验证壳聚糖的自发荧光机理,以顺-2-甲基-2-丁烯醛和2-氨基-2-甲基丙烷为原料,在-14℃无水无氧条件下合成了具有-C==N-C==C-结构单元的产物,通过傅立叶红外光谱和液质联用技术进行结构确认,并对合成产物进行了荧光光谱扫描和激光共聚焦光谱扫描分析.结果表明,合成的物质在488nm激发下,于510～540和570～600nm波段采集到了稳定的荧光,与壳聚糖荧光微球的荧光波长一致.据此可推断壳聚糖微球的自发荧光主要源于-C==N-C==C-共轭结构单元.

英文摘要：

A compound containing -C--N-C--C- was synthesized by 2-methyl-2-crotonaldehyde and 2-amino-2-methyl propane. Its structure was confirmed by infrared spectroscopy and liquid chromatography-mass spectroscopy. And the results showed that a relatively pure compound was obtained. Furthermore, its fluorescent property was analyzed by fluorescence spectroscopy and laser scanning confocal microscope, which showed the same fluorescent property with chitosan microspheres. Therefore, the conjugating structure of -C--N-C--C- was mainly attributed to auto-fluorescence of chitosan microspheres crosslinked with glutaraldehyde.