预告的处理方法用于厌氧的污泥的酸,碱,热吃惊, KNO 3 和控制为他们有选择地充实海洋的生产氢的混合微植物群的能力被评估。海水文化媒介被用作底层。pretreated 的氢收益微植物群比 un-pretreated 控制的高(P 【 0.05 ) 。在方法学习了的预告的处理之中,热吃惊预告的处理产出最大的氢生产,它是乘控制的的 14.6。当热吃惊 pretreated 样品的氢生产上的起始的 pH 的效果被学习时,氢在全部 pH 范围上被生产(pH 4 10 ) 。氢收益在起始的 pH 达到顶点 8 (79 mL/g 蔗糖) 然后当起始的 pH 增加了,稳定地减少了。蔗糖消费在中立起始的 pH 高。在氢生产的过程期间, pH 逐渐地减少了,它显示获得的微植物群由引起酸化的细菌组成了。
Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Seawater culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P 〈 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4 -- 10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose consumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria.