中文摘要：

采用细胞酶解技术获得长石莼（缘管浒苔）原生质体，再通过细胞培养观察原生质体再生以及各种分化发育途径。结果表明，长石莼（缘管浒苔）主要存在3种分化发育途径：1）部分原生质体可直接分裂形成有假根或无假根得单细胞苗。2）部分原生质体分裂成规则细胞团或不规则细胞团，最后形成苗簇。3）部分原生质体可发育为孢子囊／配子囊，其中部分孢子囊／配子囊成熟后释放出游孢子／配子，可直接形成小苗。雌雄配子以正面结合（大部分）也可以首尾交错结合（少部分）形成合子并萌发形成小苗。研究表明，1个叶片营养细胞可形成1棵细胞苗，也可以通过孢子囊或配子囊途径形成8—32棵孢子／配子苗，说明长石莼（缘管浒苔）叶片细胞具有很强的繁殖力，这为绿潮藻暴发起因及生活史深入研究奠定了一定基础。

英文摘要：

In this paper, cell regeneration, differentiation and development of Ulva linza protoplasts were studied. In- dividual cells were enzymatically isolated from Ulva linza blades, and the cells and protoplasts were cultured and regenerated into young plants. The results showed there were three main differentiations and development pathways for isolated cells： 1） Partial protoplasts directly divided and formed the single cell seedlings with or without a rhizoid; 2） Partial protoplast divided into cell aggregates including irregular and regular cell aggregates; both could form seedlings directly. 3） Partial protoplasts divided and developed into zoosporangia or gametangia. Zoosporangia could release zoospores and then germinated into young sporelings. Gametangia could release gametes, and male and female gametes conjugated with head-tail style or tail-tail style into one zygote, which could germinate into seedling. Usually, one somatic cell in blade could form into one single-cell seedling or 8-32 sporelings through zoosporangium or gametangium development way. It was found that the cells in Ulva linza had a great reproductive search on green tide blooming and the life history of Ulva linza.