中文摘要：

目的：探讨溶瘤腺病毒（ZD55-gene）作为载体携带外源抗癌基因（黝州）抗肝癌移植瘤的生长及其安全性。方法：抽提溶瘤腺病毒ZD55-XAF1的基因组DNA，PCR扩增鉴定病毒；细菌平板培养和支原体检测试剂盒检测细胞有无细菌、支原体污染；通过荷瘤小鼠动物实验，观察溶瘤腺病毒ZD55-XAF1对肝癌移植瘤生长的抑制、小鼠的临床反应指标、血清肝毒性指标、各脏器组织中的病毒残留分布及病理切片观察。结果：细胞培养过程无细菌和支原体污染；较对照组，受试小鼠血清肝酶AST活性上升（P〈0．05），而ALT和ALP活性基本无变化（P〉0．05）；PCR检测各脏器均有病毒基因组DNA存在；HE染色显示受试小鼠各脏器具有不同程度的损伤，病毒处理对肿瘤细胞具有明显的杀伤效果，而受试小鼠的临床反应并无明显异常。结论：溶瘤腺病毒ZD55-XAF1能够抑制肿瘤生长，杀死肿瘤细胞，对小鼠血清肝酶活性影响较小而对各脏器有不同程度的轻微损伤，作为癌症基因治疗载体有潜在的应用价值但其安全性还有待提高。

英文摘要：

Purpose：To investigate the safety in oncolytic adenovirus （ZD55-gene） as a vector, introducing antitumor gene （ XAF1 ） at the process of cancer therapy. Methods ： Genomic DNA of oncolytic adenovirus ZD55- XAF1 was extracted and PCR was used to identify the ZD55-XAF1;To measure the bacteria and inycoplasma contamination during cell culture, supernatant was cultivated in LB solid medium and mycoplasma was tested by detection kits; After intravenous injection of ZD55-XAF1, several indicators were evaluated, including antitumor activity of ZD55-XAF1, clinical responses of the mice, levels of serum enzymes ALT, AST and ALP, residual and distribution of the virus in tissues, HE staining of various organs. Results ： No contamination was found during cell culture, which were used for virus amplification. Except the AST, the levels of serum enzymes were normal（ P 〉 0.05）. Residual and distribution of the virus in tissue stayed normal. But HE staining showed damage on mice organs and tumor was suppressed. Conclusion：Oncolytic adenovirus ZD55-XAF1 can effectively suppress tumor growth and viability of tumor cells with little impact on serum enzymes . But hematoxylin and eosin （HE） staining showed different degrees of damage on mice organs. Oncolytic adenovirus as a vector for cancer gene therapy has potential applied value but its safety needs to be improved.