在自然条件下,作者对机敏异漏斗蛛求偶交配和生活史进行了长期野外观察。机敏异漏斗蛛雄蛛在靠近雌蛛之前,雄蛛在距雌蛛漏斗网口约1-2cm范围内左右来回爬动,爬动过程中腹部左右摇晃,伴有腹部点击蛛网的动作,有时用触肢触网。如此反复,直至雌蛛表现性接受行为,交配开始。雄蛛完整交配1次,需要5小时左右。机敏异漏斗蛛在武汉一年发生1代,主要以卵越冬,3月份幼蛛出现,8月份可见成蛛,9月份是该蛛交配高峰。
Before approaching Allagelena difficilis females, males display a series of complex courtship behavior, including abdomen and pedipalps vibrating rhythmically. After females accept the courtship vibrations males send, females exhibit sexual receptivity and males climb female back to copulate. A complete mating lasts about 5 hours. There is one generation a year of A. difficilis in Wuhan, and eggs overwinter. Spideflings ofA. difficilis emerge in March. In August, some A. difficilis mature, and September is the mating peak of the funnel-web spider.